Search Results

Overview

Uniprot IDQ9NRY4
Protein NameRho GTPase-activating protein 35
Gene NameARHGAP35
OrganismHomo sapiens

Kla Sites from experimental identification

Position Flanking peptide
1115 PHDSTQGKIITIRNI
587 RPSDRNQKNSLSDPN

Function

Rho GTPase-activating protein (GAP) (PubMed:19673492, PubMed:28894085). Binds several acidic phospholipids which inhibits the Rho GAP activity to promote the Rac GAP activity (PubMed:19673492). This binding is inhibited by phosphorylation by PRKCA (PubMed:19673492). Involved in cell differentiation as well as cell adhesion and migration, plays an important role in retinal tissue morphogenesis, neural tube fusion, midline fusion of the cerebral hemispheres and mammary gland branching morphogenesis (By similarity). Transduces signals from p21-ras to the nucleus, acting via the ras GTPase-activating protein (GAP) (By similarity). Transduces SRC-dependent signals from cell-surface adhesion molecules, such as laminin, to promote neurite outgrowth. Regulates axon outgrowth, guidance and fasciculation (By similarity). Modulates Rho GTPase-dependent F-actin polymerization, organization and assembly, is involved in polarized cell migration and in the positive regulation of ciliogenesis and cilia elongation (By similarity). During mammary gland development, is required in both the epithelial and stromal compartments for ductal outgrowth (By similarity). Represses transcription of the glucocorticoid receptor by binding to the cis-acting regulatory sequence 5'-GAGAAAAGAAACTGGAGAAACTC-3'; this function is however unclear and would need additional experimental evidences (PubMed:1894621)

Protein Sequence

10 MMMARKQDVR 20 IPTYNISVVG 30 LSGTEKEKGQ 40 CGIGKSCLCN 50 RFVRPSADEF 60 HLDHTSVLST 70 SDFGGRVVNN 80 DHFLYWGEVS 90 RSLEDCVECK 100 MHIVEQTEFI 110 DDQTFQPHRS 120 TALQPYIKRA 130 AATKLASAEK 140 LMYFCTDQLG 150 LEQDFEQKQM 160 PDGKLLVDGF 170 LLGIDVSRGM 180 NRNFDDQLKF 190 VSNLYNQLAK 200 TKKPIVVVLT 210 KCDEGVERYI 220 RDAHTFALSK 230 KNLQVVETSA 240 RSNVNVDLAF 250 STLVQLIDKS 260 RGKTKIIPYF 270 EALKQQSQQI 280 ATAKDKYEWL 290 VSRIVKNHNE 300 NWLSVSRKMQ 310 ASPEYQDYVY 320 LEGTQKAKKL 330 FLQHIHRLKH 340 EHIERRRKLY 350 LAALPLAFEA 360 LIPNLDEIDH 370 LSCIKAKKLL 380 ETKPEFLKWF 390 VVLEETPWDA 400 TSHIDNMENE 410 RIPFDLMDTV 420 PAEQLYEAHL 430 EKLRNERKRV 440 EMRRAFKENL 450 ETSPFITPGK 460 PWEEARSFIM 470 NEDFYQWLEE 480 SVYMDIYGKH 490 QKQIIDKAKE 500 EFQELLLEYS 510 ELFYELELDA 520 KPSKEKMGVI 530 QDVLGEEQRF 540 KALQKLQAER 550 DALILKHIHF 560 VYHPTKETCP 570 SCPACVDAKI 580 EHLISSRFIR 590 PSDRNQKNSL 600 SDPNIDRINL 610 VILGKDGLAR 620 ELANEIRALC 630 TNDDKYVIDG 640 KMYELSLRPI 650 EGNVRLPVNS 660 FQTPTFQPHG 670 CLCLYNSKES 680 LSYVVESIEK 690 SRESTLGRRD 700 NHLVHLPLTL 710 ILVNKRGDTS 720 GETLHSLIQQ 730 GQQIASKLQC 740 VFLDPASAGI 750 GYGRNINEKQ 760 ISQVLKGLLD 770 SKRNLNLVSS 780 TASIKDLADV 790 DLRIVMCLMC 800 GDPFSADDIL 810 FPVLQSQTCK 820 SSHCGSNNSV 830 LLELPIGLHK 840 KRIELSVLSY 850 HSSFSIRKSR 860 LVHGYIVFYS 870 AKRKASLAML 880 RAFLCEVQDI 890 IPIQLVALTD 900 GAVDVLDNDL 910 SREQLTEGEE 920 IAQEIDGRFT 930 SIPCSQPQHK 940 LEIFHPFFKD 950 VVEKKNIIEA 960 THMYDNAAEA 970 CSTTEEVFNS 980 PRAGSPLCNS 990 NLQDSEEDIE 1000 PSYSLFREDT 1010 SLPSLSKDHS 1020 KLSMELEGND 1030 GLSFIMSNFE 1040 SKLNNKVPPP 1050 VKPKPPVHFE 1060 ITKGDLSYLD 1070 QGHRDGQRKS 1080 VSSSPWLPQD 1090 GFDPSDYAEP 1100 MDAVVKPRNE 1110 EENIYSVPHD 1120 STQGKIITIR 1130 NINKAQSNGS 1140 GNGSDSEMDT 1150 SSLERGRKVS 1160 IVSKPVLYRT 1170 RCTRLGRFAS 1180 YRTSFSVGSD 1190 DELGPIRKKE 1200 EDQASQGYKG 1210 DNAVIPYETD 1220 EDPRRRNILR 1230 SLRRNTKKPK 1240 PKPRPSITKA 1250 TWESNYFGVP 1260 LTTVVTPEKP 1270 IPIFIERCIE 1280 YIEATGLSTE 1290 GIYRVSGNKS 1300 EMESLQRQFD 1310 QDHNLDLAEK 1320 DFTVNTVAGA 1330 MKSFFSELPD 1340 PLVPYNMQID 1350 LVEAHKINDR 1360 EQKLHALKEV 1370 LKKFPKENHE 1380 VFKYVISHLN 1390 KVSHNNKVNL 1400 MTSENLSICF 1410 WPTLMRPDFS 1420 TMDALTATRT 1430 YQTIIELFIQ 1440 QCPFFFYNRP 1450 ITEPPGARPS 1460 SPSAVASTVP 1470 FLTSTPVTSQ 1480 PSPPQSPPPT 1490 PQSPMQPLLP SQLQAEHTL

Gene Ontology

Classification GO ID Description
Cellular Component GO:0015629 actin cytoskeleton
Cellular Component GO:0036064 ciliary basal body
Cellular Component GO:0005829 cytosol
Cellular Component GO:0005634 nucleus
Cellular Component GO:0005886 plasma membrane
Molecular Function GO:0003677 DNA binding
Molecular Function GO:0005525 GTP binding
Molecular Function GO:0005096 GTPase activator activity
Molecular Function GO:0003924 GTPase activity
Molecular Function GO:0005543 phospholipid binding
Biological Process GO:0007411 axon guidance
Biological Process GO:0007413 axonal fasciculation
Biological Process GO:0016477 cell migration
Biological Process GO:0021955 central nervous system neuron axonogenesis
Biological Process GO:0006351 DNA-templated transcription
Biological Process GO:0030950 establishment or maintenance of actin cytoskeleton polarity
Biological Process GO:0030879 mammary gland development
Biological Process GO:0035024 negative regulation of Rho protein signal transduction
Biological Process GO:0097485 neuron projection guidance
Biological Process GO:0045724 positive regulation of cilium assembly
Biological Process GO:0010976 positive regulation of neuron projection development
Biological Process GO:0032956 regulation of actin cytoskeleton organization
Biological Process GO:0008064 regulation of actin polymerization or depolymerization
Biological Process GO:0050770 regulation of axonogenesis
Biological Process GO:0008361 regulation of cell size
Biological Process GO:0051056 regulation of small GTPase mediated signal transduction
Biological Process GO:0007266 Rho protein signal transduction
Biological Process GO:0044319 wound healing, spreading of cells

Reference

[1] Yang Z, Yan C, Ma J, Peng P, Ren X et al.. Lactylome analysis suggests lactylation-dependent mechanisms of metabolic adaptation in hepatocellular carcinoma.. Nat Metab 5(1):61-79. 2023 Jan. PMID: 36593272.