Search Results

Overview

Uniprot IDQ9NVP1
Protein NameATP-dependent RNA helicase DDX18
Gene NameDDX18
OrganismHomo sapiens

Kla Sites from experimental identification

Position Flanking peptide
116 KKKKKKRKMVNDAEP
571 QLEKLIEKNYFLHKS
645 GGGFGYQKTKKVEKS
656 VEKSKIFKHISKKSS
89 KVTKSPQKSTVLTNG

Function

ATP-dependent RNA helicase that plays a role in the regulation of R-loop homeostasis in both endogenous R-loop-prone regions and at sites of DNA damage. At endogenous loci such as actively transcribed genes, may act as a helicase to resolve the formation of R-loop during transcription and prevent the interference of R-loop with DNA-replication machinery. Also participates in the removal of DNA-lesion-associated R-loop (PubMed:35858569). Plays an essential role for establishing pluripotency during embryogenesis and for pluripotency maintenance in embryonic stem cells. Mechanistically, prevents the polycomb repressive complex 2 (PRC2) from accessing rDNA loci and protects the active chromatin status in nucleolus (By similarity)

Protein Sequence

10 MSHLPMKLLR 20 KKIEKRNLKL 30 RQRNLKFQGA 40 SNLTLSETQN 50 GDVSEETMGS 60 RKVKKSKQKP 70 MNVGLSETQN 80 GGMSQEAVGN 90 IKVTKSPQKS 100 TVLTNGEAAM 110 QSSNSESKKK 120 KKKKRKMVND 130 AEPDTKKAKT 140 ENKGKSEEES 150 AETTKETENN 160 VEKPDNDEDE 170 SEVPSLPLGL 180 TGAFEDTSFA 190 SLCNLVNENT 200 LKAIKEMGFT 210 NMTEIQHKSI 220 RPLLEGRDLL 230 AAAKTGSGKT 240 LAFLIPAVEL 250 IVKLRFMPRN 260 GTGVLILSPT 270 RELAMQTFGV 280 LKELMTHHVH 290 TYGLIMGGSN 300 RSAEAQKLGN 310 GINIIVATPG 320 RLLDHMQNTP 330 GFMYKNLQCL 340 VIDEADRILD 350 VGFEEELKQI 360 IKLLPTRRQT 370 MLFSATQTRK 380 VEDLARISLK 390 KEPLYVGVDD 400 DKANATVDGL 410 EQGYVVCPSE 420 KRFLLLFTFL 430 KKNRKKKLMV 440 FFSSCMSVKY 450 HYELLNYIDL 460 PVLAIHGKQK 470 QNKRTTTFFQ 480 FCNADSGTLL 490 CTDVAARGLD 500 IPEVDWIVQY 510 DPPDDPKEYI 520 HRVGRTARGL 530 NGRGHALLIL 540 RPEELGFLRY 550 LKQSKVPLSE 560 FDFSWSKISD 570 IQSQLEKLIE 580 KNYFLHKSAQ 590 EAYKSYIRAY 600 DSHSLKQIFN 610 VNNLNLPQVA 620 LSFGFKVPPF 630 VDLNVNSNEG 640 KQKKRGGGGG 650 FGYQKTKKVE 660 KSKIFKHISK 670 KSSDSRQFSH

Gene Ontology

Classification GO ID Description
Cellular Component GO:0005694 chromosome
Cellular Component GO:0016020 membrane
Cellular Component GO:0005730 nucleolus
Molecular Function GO:0005524 ATP binding
Molecular Function GO:0016887 ATP hydrolysis activity
Molecular Function GO:0003723 RNA binding
Molecular Function GO:0003724 RNA helicase activity
Biological Process GO:0071392 cellular response to estradiol stimulus
Biological Process GO:0000463 maturation of LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)

Reference

[1] Yang D, Yin J, Shan L, Yi X, Zhang W et al.. Identification of lysine-lactylated substrates in gastric cancer cells.. iScience 25(7):104630. 2022 Jul 15. PMID: 35800753.

[2] Yang Z, Yan C, Ma J, Peng P, Ren X et al.. Lactylome analysis suggests lactylation-dependent mechanisms of metabolic adaptation in hepatocellular carcinoma.. Nat Metab 5(1):61-79. 2023 Jan. PMID: 36593272.

[3] He C, Zhang J, Bai X, Lu C, Zhang K. Lysine lactylation-based insight to understanding the characterization of cervical cancer.. Biochim Biophys Acta Mol Basis Dis 1870(7):167356. 2024 Oct. PMID: 39025375.

[4] Bao Q, Wan N, He Z, Cao J, Yuan W et al.. Subcellular Proteomic Mapping of Lysine Lactylation.. J Am Soc Mass Spectrom 35(12):3221-3232. 2024 Dec 4. PMID: 39569522.

[5] Wu Q, Li Z, Gong T, Zheng X, Zhou X et al.. Porphyromonas gingivalis infection induces lysine lactylation reprogramming in human umbilical vein endothelial cells.. Front Cell Infect Microbiol 16:1706727. 2026. PMID: 41696360.