Overview
| Uniprot ID | Q9NVU0 |
|---|
| Protein Name | DNA-directed RNA polymerase III subunit RPC5 |
|---|
| Gene Name | POLR3E |
|---|
| Organism | Homo sapiens |
|---|
Kla Sites from experimental identification
| Position |
Flanking peptide |
| 467 |
RIQVAKTKAQQNHAL |
Function
DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates (PubMed:12391170, PubMed:20413673, PubMed:35637192). Specific peripheric component of RNA polymerase III (Pol III) which synthesizes small non-coding RNAs including 5S rRNA, snRNAs, tRNAs and miRNAs from at least 500 distinct genomic loci. Assembles with POLR3D/RPC4 forming a subcomplex that binds the Pol III core. Enables recruitment of Pol III at transcription initiation site and drives transcription initiation from both type 2 and type 3 DNA promoters. Required for efficient transcription termination and reinitiation (By similarity) (PubMed:12391170, PubMed:20413673, PubMed:35637192). Plays a key role in sensing and limiting infection by intracellular bacteria and DNA viruses. Acts as a nuclear and cytosolic DNA sensor involved in innate immune response. Can sense non-self dsDNA that serves as template for transcription into dsRNA. The non-self RNA polymerase III transcripts, such as Epstein-Barr virus-encoded RNAs (EBERs) induce type I interferon and NF-kappa-B through the RIG-I pathway (PubMed:19609254, PubMed:19631370)
Protein Sequence
10
MANEEDDPVV
20
QEIDVYLAKS
30
LAEKLYLFQY
40
PVRPASMTYD
50
DIPHLSAKIK
60
PKQQKVELEM
70
AIDTLNPNYC
80
RSKGEQIALN
90
VDGACADETS
100
TYSSKLMDKQ
110
TFCSSQTTSN
120
TSRYAAALYR
130
QGELHLTPLH
140
GILQLRPSFS
150
YLDKADAKHR
160
EREAANEAGD
170
SSQDEAEDDV
180
KQITVRFSRP
190
ESEQARQRRV
200
QSYEFLQKKH
210
AEEPWVHLHY
220
YGLRDSRSEH
230
ERQYLLCPGS
240
SGVENTELVK
250
SPSEYLMMLM
260
PPSQEEEKDK
270
PVAPSNVLSM
280
AQLRTLPLAD
290
QIKILMKNVK
300
VMPFANLMSL
310
LGPSIDSVAV
320
LRGIQKVAML
330
VQGNWVVKSD
340
ILYPKDSSSP
350
HSGVPAEVLC
360
RGRDFVMWKF
370
TQSRWVVRKE
380
VATVTKLCAE
390
DVKDFLEHMA
400
VVRINKGWEF
410
ILPYDGEFIK
420
KHPDVVQRQH
430
MLWTGIQAKL
440
EKVYNLVKET
450
MPKKPDAQSG
460
PAGLVCGDQR
470
IQVAKTKAQQ
480
NHALLERELQ
490
RRKEQLRVPA
500
VPPGVRIKEE
510
PVSEEGEEDE
520
EQEAEEEPMD
530
TSPSGLHSKL
540
ANGLPLGRAA
550
GTDSFNGHPP
560
QGCASTPVAR
570
ELKAFVEATF
580
QRQFVLTLSE
590
LKRLFNLHLA
600
SLPPGHTLFS
610
GISDRMLQDT
620
VLAAGCKQIL
630
VPFPPQTAAS
640
PDEQKVFALW
650
ESGDMSDQHR
660
QVLLEIFSKN
670
YRVRRNMIQS
680
RLTQECGEDL
690
SKQEVDKVLK
700
DCCVSYGGMW
YLKGTVQS
Gene Ontology
| Classification |
GO ID |
Description |
| Cellular Component |
GO:0005829 |
cytosol |
| Cellular Component |
GO:0005654 |
nucleoplasm |
| Cellular Component |
GO:0005634 |
nucleus |
| Cellular Component |
GO:0005666 |
RNA polymerase III complex |
| Biological Process |
GO:0051607 |
defense response to virus |
| Biological Process |
GO:0045087 |
innate immune response |
| Biological Process |
GO:0006386 |
termination of RNA polymerase III transcription |
| Biological Process |
GO:0006383 |
transcription by RNA polymerase III |
| Biological Process |
GO:0006384 |
transcription initiation at RNA polymerase III promoter |
Reference
PMID: N/A