Search Results

Overview

Uniprot IDQ9NXG2
Protein NameTHUMP domain-containing protein 1
Gene NameTHUMPD1
OrganismHomo sapiens

Kla Sites from experimental identification

Position Flanking peptide
15 TTQPGGGKRKGKAQY
19 GGGKRKGKAQYVLAK
26 KAQYVLAKRARRCDA
272 QEVVKSPKDPSQLNS
280 DPSQLNSKQGNGKEA
288 QGNGKEAKLESADKS

Function

Functions as a tRNA-binding adapter to mediate NAT10-dependent tRNA acetylation modifying cytidine to N4-acetylcytidine (ac4C) (PubMed:25653167, PubMed:35196516)

Protein Sequence

10 MAAPAQQTTQ 20 PGGGKRKGKA 30 QYVLAKRARR 40 CDAGGPRQLE 50 PGLQGILITC 60 NMNERKCVEE 70 AYSLLNEYGD 80 DMYGPEKFTD 90 KDQQPSGSEG 100 EDDDAEAALK 110 KEVGDIKAST 120 EMRLRRFQSV 130 ESGANNVVFI 140 RTLGIEPEKL 150 VHHILQDMYK 160 TKKKKTRVIL 170 RMLPISGTCK 180 AFLEDMKKYA 190 ETFLEPWFKA 200 PNKGTFQIVY 210 KSRNNSHVNR 220 EEVIRELAGI 230 VCTLNSENKV 240 DLTNPQYTVV 250 VEIIKAVCCL 260 SVVKDYMLFR 270 KYNLQEVVKS 280 PKDPSQLNSK 290 QGNGKEAKLE 300 SADKSDQNNT 310 AEGKNNQQVP 320 ENTEELGQTK 330 PTSNPQVVNE 340 GGAKPELASQ 350 ATEGSKSNEN DFS

Gene Ontology

Classification GO ID Description
Cellular Component GO:0005654 nucleoplasm
Molecular Function GO:0003723 RNA binding
Biological Process GO:0006400 tRNA modification

Reference

[1] Yang D, Yin J, Shan L, Yi X, Zhang W et al.. Identification of lysine-lactylated substrates in gastric cancer cells.. iScience 25(7):104630. 2022 Jul 15. PMID: 35800753.

[2] Yang Z, Yan C, Ma J, Peng P, Ren X et al.. Lactylome analysis suggests lactylation-dependent mechanisms of metabolic adaptation in hepatocellular carcinoma.. Nat Metab 5(1):61-79. 2023 Jan. PMID: 36593272.

[3] Lin Y, Chen M, Wang D, Yu Y, Chen R et al.. Multi-Proteomic Analysis Reveals the Effect of Protein Lactylation on Matrix and Cholesterol Metabolism in Tendinopathy.. J Proteome Res 22(6):1712-1722. 2023 Jun 2. PMID: 37159428.

[4] Yang YH, Wang QC, Kong J, Yang JT, Liu JF. Global profiling of lysine lactylation in human lungs.. Proteomics 23(15):e2200437. 2023 Aug. PMID: 37170646.

[5] Cheng Z, Huang H, Li M, Chen Y. Proteomic analysis identifies PFKP lactylation in SW480 colon cancer cells.. iScience 27(1):108645. 2024 Jan 19. PMID: 38155775.

[6] He C, Zhang J, Bai X, Lu C, Zhang K. Lysine lactylation-based insight to understanding the characterization of cervical cancer.. Biochim Biophys Acta Mol Basis Dis 1870(7):167356. 2024 Oct. PMID: 39025375.

[7] Bao Q, Wan N, He Z, Cao J, Yuan W et al.. Subcellular Proteomic Mapping of Lysine Lactylation.. J Am Soc Mass Spectrom 35(12):3221-3232. 2024 Dec 4. PMID: 39569522.

[8] Shi CM, Wang QC, Li XL, Yang YH, Tang XY et al.. Global Profiling of Protein Lactylation in Human Hippocampi.. Proteomics Clin Appl 19(2):e202400061. 2025 Mar. PMID: 39610256.

[9] Guo X, Ren X, Yan C, Huang H. Quantitative Proteomics Reveals the Role of Lysine Lactylation in Lenalidomide-Resistance in Multiple Myeloma Cells.. ACS Chem Biol 20(7):1728-1738. 2025 Jul 18. PMID: 40590393.

[10] He J, Lai T, Zhou Z, Yang H, Lei Z et al.. Multiomics profiling reveals the involvement of protein lactylation in nonhomologous end joining pathway conferring radioresistance in lung adenocarcinoma cell.. Sci Rep 15(1):24651. 2025 Jul 9. PMID: 40634431.

[11] Wu Q, Li Z, Gong T, Zheng X, Zhou X et al.. Porphyromonas gingivalis infection induces lysine lactylation reprogramming in human umbilical vein endothelial cells.. Front Cell Infect Microbiol 16:1706727. 2026. PMID: 41696360.