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Overview

Uniprot ID	Q9NXL9
Protein Name	DNA helicase MCM9
Gene Name	MCM9
Organism	Homo sapiens

Kla Sites from experimental identification

Position	Flanking peptide
911	EPAIENVKPPGSPVA
919	PPGSPVAKLAKFTFK
922	SPVAKLAKFTFKQKS

Function

Component of the MCM8-MCM9 complex, which is involved in the repair of double-stranded DNA breaks (DBSs) and DNA interstrand cross-links (ICLs) by homologous recombination (HR) (PubMed:23401855). The MCM8-MCM9 complex is a 3'-5' DNA helicase and single-stranded (ss)DNA-stimulated ATPase which binds ssDNA in the presence of nucleoside triphosphates (PubMed:37309874). Required for DNA resection by the MRE11-RAD50-NBN/NBS1 (MRN) complex by recruiting the MRN complex to the repair site and by promoting the complex nuclease activity (PubMed:26215093). Indirectly regulates the recruitment of downstream effector RAD51 to DNA damage sites including DBSs and ICLs, probably by regulating the localization of the MNR complex (PubMed:23401855). Acts as a helicase in DNA mismatch repair (MMR) following DNA replication errors to unwind the mismatch containing DNA strand (PubMed:26300262). In addition, recruits MLH1, a component of the MMR complex, to chromatin (PubMed:26300262). The MCM8-MCM9 complex is dispensable for DNA replication and S phase progression (PubMed:23401855). Plays a key role during gametogenesis, probably by regulating HR (By similarity)

Protein Sequence

10 MNSDQVTLVG 20 QVFESYVSEY 30 HKNDILLILK 40 ERDEDAHYPV 50 VVNAMTLFET 60 NMEIGEYFNM 70 FPSEVLTIFD 80 SALRRSALTI 90 LQSLSQPEAV 100 SMKQNLHARI 110 SGLPVCPELV 120 REHIPKTKDV 130 GHFLSVTGTV 140 IRTSLVKVLE 150 FERDYMCNKC 160 KHVFVIKADF 170 EQYYTFCRPS 180 SCPSLESCDS 190 SKFTCLSGLS 200 SSPTRCRDYQ 210 EIKIQEQVQR 220 LSVGSIPRSM 230 KVILEDDLVD 240 SCKSGDDLTI 250 YGIVMQRWKP 260 FQQDVRCEVE 270 IVLKANYIQV 280 NNEQSSGIIM 290 DEEVQKEFED 300 FWEYYKSDPF 310 AGRNVILASL 320 CPQVFGMYLV 330 KLAVAMVLAG 340 GIQRTDATGT 350 RVRGESHLLL 360 VGDPGTGKSQ 370 FLKYAAKITP 380 RSVLTTGIGS 390 TSAGLTVTAV 400 KDSGEWNLEA 410 GALVLADAGL 420 CCIDEFNSLK 430 EHDRTSIHEA 440 MEQQTISVAK 450 AGLVCKLNTR 460 TTILAATNPK 470 GQYDPQESVS 480 VNIALGSPLL 490 SRFDLILVLL 500 DTKNEDWDRI 510 ISSFILENKG 520 YPSKSEKLWS 530 MEKMKTYFCL 540 IRNLQPTLSD 550 VGNQVLLRYY 560 QMQRQSDCRN 570 AARTTIRLLE 580 SLIRLAEAHA 590 RLMFRDTVTL 600 EDAITVVSVM 610 ESSMQGGALL 620 GGVNALHTSF 630 PENPGEQYQR 640 QCELILEKLE 650 LQSLLSEELR 660 RLERLQNQSV 670 HQSQPRVLEV 680 ETTPGSLRNG 690 PGEESNFRTS 700 SQQEINYSTH 710 IFSPGGSPEG 720 SPVLDPPPHL 730 EPNRSTSRKH 740 SAQHKNNRDD 750 SLDWFDFMAT 760 HQSEPKNTVV 770 VSPHPKTSGE 780 NMASKISNST 790 SQGKEKSEPG 800 QRSKVDIGLL 810 PSPGETGVPW 820 RADNVESNKK 830 KRLALDSEAA 840 VSADKPDSVL 850 THHVPRNLQK 860 LCKERAQKLC 870 RNSTRVPAQC 880 TVPSHPQSTP 890 VHSPDRMLDS 900 PKRKRPKSLA 910 QVEEPAIENV 920 KPPGSPVAKL 930 AKFTFKQKSK 940 LIHSFEDHSH 950 VSPGATKIAV 960 HSPKISQRRT 970 RRDAALPVKR 980 PGKLTSTPGN 990 QISSQPQGET 1000 KEVSQQPPEK 1010 HGPREKVMCA 1020 PEKRIIQPEL 1030 ELGNETGCAH 1040 LTCEGDKKEE 1050 VSGSNKSGKV 1060 HACTLARLAN 1070 FCFTPPSESK 1080 SKSPPPERKN 1090 RGERGPSSPP 1100 TTTAPMRVSK 1110 RKSFQLRGST 1120 EKLIVSKESL 1130 FTLPELGDEA 1140 FDCDWDEEMR KKS

Gene Ontology

Classification	GO ID	Description
Cellular Component	GO:0097362	MCM8-MCM9 complex
Cellular Component	GO:0005694	chromosome
Cellular Component	GO:0042555	MCM complex
Cellular Component	GO:0005634	nucleus
Molecular Function	GO:0043138	3'-5' DNA helicase activity
Molecular Function	GO:0005524	ATP binding
Molecular Function	GO:0016887	ATP hydrolysis activity
Molecular Function	GO:0003682	chromatin binding
Molecular Function	GO:0003678	DNA helicase activity
Molecular Function	GO:0019899	enzyme binding
Molecular Function	GO:0032406	MutLbeta complex binding
Molecular Function	GO:0032407	MutSalpha complex binding
Molecular Function	GO:0032408	MutSbeta complex binding
Molecular Function	GO:0044877	protein-containing complex binding
Molecular Function	GO:0003697	single-stranded DNA binding
Biological Process	GO:0006974	DNA damage response
Biological Process	GO:0000724	double-strand break repair via homologous recombination
Biological Process	GO:0007292	female gamete generation
Biological Process	GO:0070716	mismatch repair involved in maintenance of fidelity involved in DNA-dependent DNA replication
Biological Process	GO:0071168	protein localization to chromatin
Biological Process	GO:0036298	recombinational interstrand cross-link repair

Reference

[1] He C, Zhang J, Bai X, Lu C, Zhang K. Lysine lactylation-based insight to understanding the characterization of cervical cancer.. Biochim Biophys Acta Mol Basis Dis 1870(7):167356. 2024 Oct. PMID: 39025375.