Overview
| Uniprot ID | Q9NZM5 |
|---|
| Protein Name | Ribosome biogenesis protein NOP53 |
|---|
| Gene Name | NOP53 |
|---|
| Organism | Homo sapiens |
|---|
Kla Sites from experimental identification
| Position |
Flanking peptide |
| 94 |
FFVDTGSKEKGLTKK |
| 96 |
VDTGSKEKGLTKKRT |
Function
Nucleolar protein which is involved in the integration of the 5S RNP into the ribosomal large subunit during ribosome biogenesis (PubMed:24120868). In ribosome biogenesis, may also play a role in rRNA transcription (PubMed:27729611). Also functions as a nucleolar sensor that regulates the activation of p53/TP53 in response to ribosome biogenesis perturbation, DNA damage and other stress conditions (PubMed:21741933, PubMed:24120868, PubMed:27829214). DNA damage or perturbation of ribosome biogenesis disrupt the interaction between NOP53 and RPL11 allowing RPL11 transport to the nucleoplasm where it can inhibit MDM2 and allow p53/TP53 activation (PubMed:24120868, PubMed:27829214). It may also positively regulate the function of p53/TP53 in cell cycle arrest and apoptosis through direct interaction, preventing its MDM2-dependent ubiquitin-mediated proteasomal degradation (PubMed:22522597). Originally identified as a tumor suppressor, it may also play a role in cell proliferation and apoptosis by positively regulating the stability of PTEN, thereby antagonizing the PI3K-AKT/PKB signaling pathway (PubMed:15355975, PubMed:16971513, PubMed:27729611). May also inhibit cell proliferation and increase apoptosis through its interaction with NF2 (PubMed:21167305). May negatively regulate NPM1 by regulating its nucleoplasmic localization, oligomerization and ubiquitin-mediated proteasomal degradation (PubMed:25818168). Thereby, may prevent NPM1 interaction with MYC and negatively regulate transcription mediated by the MYC-NPM1 complex (PubMed:25956029). May also regulate cellular aerobic respiration (PubMed:24556985). In the cellular response to viral infection, may play a role in the attenuation of interferon-beta through the inhibition of RIGI (PubMed:27824081)
Protein Sequence
10
MAAGGSGVGG
20
KRSSKSDADS
30
GFLGLRPTSV
40
DPALRRRRRG
50
PRNKKRGWRR
60
LAQEPLGLEV
70
DQFLEDVRLQ
80
ERTSGGLLSE
90
APNEKLFFVD
100
TGSKEKGLTK
110
KRTKVQKKSL
120
LLKKPLRVDL
130
ILENTSKVPA
140
PKDVLAHQVP
150
NAKKLRRKEQ
160
LWEKLAKQGE
170
LPREVRRAQA
180
RLLNPSATRA
190
KPGPQDTVER
200
PFYDLWASDN
210
PLDRPLVGQD
220
EFFLEQTKKK
230
GVKRPARLHT
240
KPSQAPAVEV
250
APAGASYNPS
260
FEDHQTLLSA
270
AHEVELQRQK
280
EAEKLERQLA
290
LPATEQAATQ
300
ESTFQELCEG
310
LLEESDGEGE
320
PGQGEGPEAG
330
DAEVCPTPAR
340
LATTEKKTEQ
350
QRRREKAVHR
360
LRVQQAALRA
370
ARLRHQELFR
380
LRGIKAQVAL
390
RLAELARRQR
400
RRQARREAEA
410
DKPRRLGRLK
420
YQAPDIDVQL
430
SSELTDSLRT
440
LKPEGNILRD
450
RFKSFQRRNM
460
IEPRERAKFK
470
RKYKVKLVEK
RAFREIQL
Gene Ontology
| Classification |
GO ID |
Description |
| Cellular Component |
GO:0005829 |
cytosol |
| Cellular Component |
GO:0001650 |
fibrillar center |
| Cellular Component |
GO:0005730 |
nucleolus |
| Cellular Component |
GO:0005654 |
nucleoplasm |
| Molecular Function |
GO:0008097 |
5S rRNA binding |
| Molecular Function |
GO:0042802 |
identical protein binding |
| Molecular Function |
GO:0002039 |
p53 binding |
| Molecular Function |
GO:0003723 |
RNA binding |
| Biological Process |
GO:0071456 |
cellular response to hypoxia |
| Biological Process |
GO:0006974 |
DNA damage response |
| Biological Process |
GO:0006281 |
DNA repair |
| Biological Process |
GO:0007095 |
mitotic G2 DNA damage checkpoint signaling |
| Biological Process |
GO:0051898 |
negative regulation of phosphatidylinositol 3-kinase/protein kinase B signal transduction |
| Biological Process |
GO:0032435 |
negative regulation of proteasomal ubiquitin-dependent protein catabolic process |
| Biological Process |
GO:0031333 |
negative regulation of protein-containing complex assembly |
| Biological Process |
GO:1901797 |
negative regulation of signal transduction by p53 class mediator |
| Biological Process |
GO:0000122 |
negative regulation of transcription by RNA polymerase II |
| Biological Process |
GO:1901837 |
negative regulation of transcription of nucleolar large rRNA by RNA polymerase I |
| Biological Process |
GO:0032436 |
positive regulation of proteasomal ubiquitin-dependent protein catabolic process |
| Biological Process |
GO:1903006 |
positive regulation of protein K63-linked deubiquitination |
| Biological Process |
GO:1902570 |
protein localization to nucleolus |
| Biological Process |
GO:1990173 |
protein localization to nucleoplasm |
| Biological Process |
GO:0050821 |
protein stabilization |
| Biological Process |
GO:1903715 |
regulation of aerobic respiration |
| Biological Process |
GO:0042981 |
regulation of apoptotic process |
| Biological Process |
GO:0051726 |
regulation of cell cycle |
| Biological Process |
GO:0001932 |
regulation of protein phosphorylation |
| Biological Process |
GO:0039535 |
regulation of RIG-I signaling pathway |
| Biological Process |
GO:1901796 |
regulation of signal transduction by p53 class mediator |
| Biological Process |
GO:0000027 |
ribosomal large subunit assembly |
| Biological Process |
GO:0006364 |
rRNA processing |
Reference
PMID: N/A