Search Results

Overview

Uniprot ID	Q9P035
Protein Name	Very-long-chain (3R)-3-hydroxyacyl-CoA dehydratase 3
Gene Name	HACD3
Organism	Homo sapiens

Kla Sites from experimental identification

Position	Flanking peptide
84	QVNITVQKKVSQWWE
85	VNITVQKKVSQWWER

Function

Catalyzes the third of the four reactions of the long-chain fatty acids elongation cycle. This endoplasmic reticulum-bound enzymatic process, allows the addition of two carbons to the chain of long- and very long-chain fatty acids/VLCFAs per cycle. This enzyme catalyzes the dehydration of the 3-hydroxyacyl-CoA intermediate into trans-2,3-enoyl-CoA, within each cycle of fatty acid elongation. Thereby, it participates in the production of VLCFAs of different chain lengths that are involved in multiple biological processes as precursors of membrane lipids and lipid mediators. May be involved in Rac1-signaling pathways leading to the modulation of gene expression. Promotes insulin receptor/INSR autophosphorylation and is involved in INSR internalization (PubMed:25687571)

Protein Sequence

10 MENQVLTPHV 20 YWAQRHRELY 30 LRVELSDVQN 40 PAISITENVL 50 HFKAQGHGAK 60 GDNVYEFHLE 70 FLDLVKPEPV 80 YKLTQRQVNI 90 TVQKKVSQWW 100 ERLTKQEKRP 110 LFLAPDFDRW 120 LDESDAEMEL 130 RAKEEERLNK 140 LRLESEGSPE 150 TLTNLRKGYL 160 FMYNLVQFLG 170 FSWIFVNLTV 180 RFCILGKESF 190 YDTFHTVADM 200 MYFCQMLAVV 210 ETINAAIGVT 220 TSPVLPSLIQ 230 LLGRNFILFI 240 IFGTMEEMQN 250 KAVVFFVFYL 260 WSAIEIFRYS 270 FYMLTCIDMD 280 WKVLTWLRYT 290 LWIPLYPLGC 300 LAEAVSVIQS 310 IPIFNETGRF 320 SFTLPYPVKI 330 KVRFSFFLQI 340 YLIMIFLGLY 350 INFRHLYKQR 360 RRRYGQKKKK IH

Gene Ontology

Classification	GO ID	Description
Cellular Component	GO:0005783	endoplasmic reticulum
Cellular Component	GO:0005789	endoplasmic reticulum membrane
Cellular Component	GO:0005925	focal adhesion
Molecular Function	GO:0018812	3-hydroxyacyl-CoA dehydratase activity
Molecular Function	GO:0019899	enzyme binding
Molecular Function	GO:0005096	GTPase activator activity
Molecular Function	GO:0102158	very-long-chain (3R)-3-hydroxyacyl-CoA dehydratase activity
Biological Process	GO:0007249	canonical NF-kappaB signal transduction
Biological Process	GO:0030497	fatty acid elongation
Biological Process	GO:1902532	negative regulation of intracellular signal transduction
Biological Process	GO:0046726	positive regulation by virus of viral protein levels in host cell
Biological Process	GO:0045070	positive regulation of viral genome replication
Biological Process	GO:0016601	Rac protein signal transduction
Biological Process	GO:0007264	small GTPase-mediated signal transduction
Biological Process	GO:0030148	sphingolipid biosynthetic process
Biological Process	GO:0042761	very long-chain fatty acid biosynthetic process

Reference

[1] Yang Z, Yan C, Ma J, Peng P, Ren X et al.. Lactylome analysis suggests lactylation-dependent mechanisms of metabolic adaptation in hepatocellular carcinoma.. Nat Metab 5(1):61-79. 2023 Jan. PMID: 36593272.

[2] He J, Lai T, Zhou Z, Yang H, Lei Z et al.. Multiomics profiling reveals the involvement of protein lactylation in nonhomologous end joining pathway conferring radioresistance in lung adenocarcinoma cell.. Sci Rep 15(1):24651. 2025 Jul 9. PMID: 40634431.