Overview
| Uniprot ID | Q9P1Y5 |
|---|
| Protein Name | Calmodulin-regulated spectrin-associated protein 3 |
|---|
| Gene Name | CAMSAP3 |
|---|
| Organism | Homo sapiens |
|---|
Kla Sites from experimental identification
| Position |
Flanking peptide |
| 634 |
KHRQRLGKSAFLQVQ |
| 812 |
DSLPHLRKFSPSQVP |
Function
Key microtubule-organizing protein that specifically binds the minus-end of non-centrosomal microtubules and regulates their dynamics and organization (PubMed:19041755, PubMed:23169647). Specifically recognizes growing microtubule minus-ends and autonomously decorates and stabilizes microtubule lattice formed by microtubule minus-end polymerization (PubMed:24486153). Acts on free microtubule minus-ends that are not capped by microtubule-nucleating proteins or other factors and protects microtubule minus-ends from depolymerization (PubMed:24486153). In addition, it also reduces the velocity of microtubule polymerization (PubMed:24486153). Required for the biogenesis and the maintenance of zonula adherens by anchoring the minus-end of microtubules to zonula adherens and by recruiting the kinesin KIFC3 to those junctional sites (PubMed:19041755). Required for orienting the apical-to-basal polarity of microtubules in epithelial cells: acts by tethering non-centrosomal microtubules to the apical cortex, leading to their longitudinal orientation (PubMed:26715742, PubMed:27802168). Plays a key role in early embryos, which lack centrosomes: accumulates at the microtubule bridges that connect pairs of cells and enables the formation of a non-centrosomal microtubule-organizing center that directs intracellular transport in the early embryo (By similarity). Couples non-centrosomal microtubules with actin: interaction with MACF1 at the minus ends of non-centrosomal microtubules, tethers the microtubules to actin filaments, regulating focal adhesion size and cell migration (PubMed:27693509). Plays a key role in the generation of non-centrosomal microtubules by accumulating in the pericentrosomal region and cooperating with KATNA1 to release non-centrosomal microtubules from the centrosome (PubMed:28386021). Through the microtubule cytoskeleton, also regulates the organization of cellular organelles including the Golgi and the early endosomes (PubMed:28089391). Through interaction with AKAP9, involved in translocation of Golgi vesicles in epithelial cells, where microtubules are mainly non-centrosomal (PubMed:28089391). Plays an important role in motile cilia function by facilitatating proper orientation of basal bodies and formation of central microtubule pairs in motile cilia (By similarity)
Protein Sequence
10
MVEAAPPGPG
20
PLRRTFLVPE
30
IKSLDQYDFS
40
RAKAAASLAW
50
VLRAAFGGAE
60
HVPPELWEPF
70
YTDQYAQEHV
80
KPPVTRLLLS
90
AELYCRAWRQ
100
ALPQLETPPN
110
PSALLALLAR
120
RGTVPALPER
130
PVREADLRHQ
140
PILMGAHLAV
150
IDALMAAFAF
160
EWTKTLPGPL
170
ALTSLEHKLL
180
FWVDTTVRRL
190
QEKTEQEAAQ
200
RASPAAPADG
210
AAPAQPSIRY
220
RKDRVVARRA
230
PCFPTVTSLQ
240
DLASGAALAA
250
TIHCYCPQLL
260
RLEEVCLKDP
270
MSVADSLYNL
280
QLVQDFCASR
290
LPRGCPLSLE
300
DLLYVPPPLK
310
VNLVVMLAEL
320
FMCFEVLKPD
330
FVQVKDLPDG
340
HAASPRGTEA
350
SPPQNNSGSS
360
SPVFTFRHPL
370
LSSGGPQSPL
380
RGSTGSLKSS
390
PSMSHMEALG
400
KAWNRQLSRP
410
LSQAVSFSTP
420
FGLDSDVDVV
430
MGDPVLLRSV
440
SSDSLGPPRP
450
APARTPTQPP
460
PEPGDLPTIE
470
EALQIIHSAE
480
PRLLPDGAAD
490
GSFYLHSPEG
500
PSKPSLASPY
510
LPEGTSKPLS
520
DRPTKAPVYM
530
PHPETPSKPS
540
PCLVGEASKP
550
PAPSEGSPKA
560
VASSPAATNS
570
EVKMTSFAER
580
KKQLVKAEAE
590
AGAGSPTSTP
600
APPEALSSEM
610
SELSARLEEK
620
RRAIEAQKRR
630
IEAIFAKHRQ
640
RLGKSAFLQV
650
QPREASGEAE
660
AEAEEADSGP
670
VPGGERPAGE
680
GQGEPTSRPK
690
AVTFSPDLGP
700
VPHEGLGEYN
710
RAVSKLSAAL
720
SSLQRDMQRL
730
TDQQQRLLAP
740
PEAPGSAPPP
750
AAWVIPGPTT
760
GPKAASPSPA
770
RRVPATRRSP
780
GPGPSQSPRS
790
PKHTRPAELR
800
LAPLTRVLTP
810
PHDVDSLPHL
820
RKFSPSQVPV
830
QTRSSILLAE
840
ETPPEEPAAR
850
PGLIEIPLGS
860
LADPAAEDEG
870
DGSPAGAEDS
880
LEEEASSEGE
890
PRVGLGFFYK
900
DEDKPEDEMA
910
QKRASLLERQ
920
QRRAEEARRR
930
KQWQEVEKEQ
940
RREEAARLAQ
950
EEAPGPAPLV
960
SAVPMATPAP
970
AARAPAEEEV
980
GPRKGDFTRQ
990
EYERRAQLKL
1000
MDDLDKVLRP
1010
RAAGSGGPGR
1020
GGRRATRPRS
1030
GCCDDSALAR
1040
SPARGLLGSR
1050
LSKIYSQSTL
1060
SLSTVANEAH
1070
NNLGVKRPTS
1080
RAPSPSGLMS
1090
PSRLPGSRER
1100
DWENGSNASS
1110
PASVPEYTGP
1120
RLYKEPSAKS
1130
NKFIIHNALS
1140
HCCLAGKVNE
1150
PQKNRILEEI
1160
EKSKANHFLI
1170
LFRDSSCQFR
1180
ALYTLSGETE
1190
ELSRLAGYGP
1200
RTVTPAMVEG
1210
IYKYNSDRKR
1220
FTQIPAKTMS
1230
MSVDAFTIQG
1240
HLWQGKKPTT
PKKGGGTPK
Gene Ontology
| Classification |
GO ID |
Description |
| Cellular Component |
GO:0005930 |
axoneme |
| Biological Process |
GO:0043111 |
replication fork arrest |
| Biological Process |
GO:0045218 |
zonula adherens maintenance |
| Cellular Component |
GO:0036064 |
ciliary basal body |
| Cellular Component |
GO:0005737 |
cytoplasm |
| Cellular Component |
GO:0036449 |
microtubule minus-end |
| Cellular Component |
GO:0031514 |
motile cilium |
| Cellular Component |
GO:0031298 |
replication fork protection complex |
| Cellular Component |
GO:0005915 |
zonula adherens |
| Molecular Function |
GO:0051015 |
actin filament binding |
| Molecular Function |
GO:0005516 |
calmodulin binding |
| Molecular Function |
GO:0003677 |
DNA binding |
| Molecular Function |
GO:0051011 |
microtubule minus-end binding |
| Molecular Function |
GO:0030507 |
spectrin binding |
| Biological Process |
GO:0003341 |
cilium movement |
| Biological Process |
GO:0000076 |
DNA replication checkpoint signaling |
| Biological Process |
GO:0009792 |
embryo development ending in birth or egg hatching |
| Biological Process |
GO:0090136 |
epithelial cell-cell adhesion |
| Biological Process |
GO:0045198 |
establishment of epithelial cell apical/basal polarity |
| Biological Process |
GO:0030951 |
establishment or maintenance of microtubule cytoskeleton polarity |
| Biological Process |
GO:0034453 |
microtubule anchoring |
| Biological Process |
GO:0000226 |
microtubule cytoskeleton organization |
| Biological Process |
GO:0031175 |
neuron projection development |
| Biological Process |
GO:0098840 |
protein transport along microtubule |
| Biological Process |
GO:0030334 |
regulation of cell migration |
| Biological Process |
GO:0051893 |
regulation of focal adhesion assembly |
| Biological Process |
GO:1903358 |
regulation of Golgi organization |
| Biological Process |
GO:0070507 |
regulation of microtubule cytoskeleton organization |
| Biological Process |
GO:0031113 |
regulation of microtubule polymerization |
| Biological Process |
GO:0033043 |
regulation of organelle organization |
Reference
[1] Wu Q, Li Z, Gong T, Zheng X, Zhou X et al.. Porphyromonas gingivalis infection induces lysine lactylation reprogramming in human umbilical vein endothelial cells.. Front Cell Infect Microbiol 16:1706727. 2026. PMID: 41696360.