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Overview

Uniprot IDQ9P1Z0
Protein NameZinc finger and BTB domain-containing protein 4
Gene NameZBTB4
OrganismHomo sapiens

Kla Sites from experimental identification

Position Flanking peptide
675 YSYKPKRKAGAAGGA

Function

Transcriptional repressor with bimodal DNA-binding specificity. Represses transcription in a methyl-CpG-dependent manner. Binds with a higher affinity to methylated CpG dinucleotides in the consensus sequence 5'-CGCG-3' but can also bind to the non-methylated consensus sequence 5'-CTGCNA-3' also known as the consensus kaiso binding site (KBS). Can also bind specifically to a single methyl-CpG pair and can bind hemimethylated DNA but with a lower affinity compared to methylated DNA (PubMed:16354688). Plays a role in postnatal myogenesis, may be involved in the regulation of satellite cells self-renewal (By similarity)

Protein Sequence

10 MPPPAEVTDP 20 SHAPAVLRQL 30 NEQRLRGLFC 40 DVTLIAGDTK 50 FPAHRSVLAA 60 SSPFFREALL 70 TSAPLPLPPA 80 TGGAAPNPAT 90 TTAASSSSSS 100 SSSSSSSSSS 110 ASSSSSSSSS 120 SPPPASPPAS 130 SPPRVLELPG 140 VPAAAFSDVL 150 NFIYSARLAL 160 PGGGGDGAAV 170 AEIGALGRRL 180 GISRLQGLGE 190 GGDAWVPPTP 200 APMATSQPEE 210 DSFGPGPRPA 220 GEWEGDRAEA 230 QAPDLQCSLP 240 RRPLPCPQCG 250 KSFIHPKRLQ 260 THEAQCRRGA 270 STRGSTGLGA 280 GGAGPGGPAG 290 VDASALPPPV 300 GFRGGPEHVV 310 KVVGGHVLYV 320 CAACERSYVT 330 LSSLKRHSNV 340 HSWRRKYPCR 350 YCEKVFALAE 360 YRTKHEVWHT 370 GERRYQCIFC 380 WETFVTYYNL 390 KTHQRAFHGI 400 SPGLLASEKT 410 PNGGYKPKLN 420 TLKLYRLLPM 430 RAAKRPYKTY 440 SQGAPEAPLS 450 PTLNTPAPVA 460 MPASPPPGPP 470 PAPEPGPPPS 480 VITFAHPAPS 490 VIVHGGSSSG 500 GGGSGTASTG 510 GSQAASVITY 520 TAPPRPPKKR 530 EYPPPPPEPA 540 ATPTSPATAV 550 SPATAAGPAM 560 ATTTEEAKGR 570 NPRAGRTLTY 580 TAKPVGGIGG 590 GGGPPTGAGR 600 GPSQLQAPPP 610 LCQITVRIGE 620 EAIVKRRISE 630 TDLRPGELSG 640 EEMEESEEDE 650 EEEDEEEEEE 660 DEEESKAGGE 670 DQLWRPYYSY 680 KPKRKAGAAG 690 GASVGGSGLP 700 RGRRPPRWRQ 710 KLERRSWEET 720 PAAESPAGRA 730 RTERRHRCGD 740 CAQTFTTLRK 750 LRKHQEAHGG 760 GSHSSRAGRR 770 PSTRFTCPHC 780 AKVCKTAAAL 790 SRHGQRHAAE 800 RPGGTPTPVI 810 AYSKGSAGTR 820 PGDVKEEAPQ 830 EMQVSSSSGE 840 AGGGSTAAEE 850 ASETASLQDP 860 IISGGEEPPV 870 VASGGSYVYP 880 PVQEFPLALI 890 GGGREPGGGR 900 GKSGSEGPVG 910 AGEGDRMEGI 920 GAAKVTFYPE 930 PYPLVYGPQL 940 LAAYPYNFSN 950 LAALPVALNM 960 VLPDEKGAGA 970 LPFLPGVFGY 980 AVNPQAAPPA 990 PPTPPPPTLP 1000 PPIPPKGEGE 1010 RAGVERTQKG DVG

Gene Ontology

Classification GO ID Description
Cellular Component GO:0005694 chromosome
Cellular Component GO:0016604 nuclear body
Cellular Component GO:0005654 nucleoplasm
Cellular Component GO:0005634 nucleus
Molecular Function GO:0000981 DNA-binding transcription factor activity, RNA polymerase II-specific
Molecular Function GO:0001227 DNA-binding transcription repressor activity, RNA polymerase II-specific
Molecular Function GO:0008327 methyl-CpG binding
Molecular Function GO:0010428 methyl-CpNpG binding
Molecular Function GO:0042803 protein homodimerization activity
Molecular Function GO:0019901 protein kinase binding
Molecular Function GO:0000978 RNA polymerase II cis-regulatory region sequence-specific DNA binding
Molecular Function GO:0000977 RNA polymerase II transcription regulatory region sequence-specific DNA binding
Molecular Function GO:0043565 sequence-specific DNA binding
Molecular Function GO:0008270 zinc ion binding
Biological Process GO:0006974 DNA damage response
Biological Process GO:0006351 DNA-templated transcription
Biological Process GO:0045892 negative regulation of DNA-templated transcription
Biological Process GO:0000122 negative regulation of transcription by RNA polymerase II
Biological Process GO:0006355 regulation of DNA-templated transcription

Reference

[1] He C, Zhang J, Bai X, Lu C, Zhang K. Lysine lactylation-based insight to understanding the characterization of cervical cancer.. Biochim Biophys Acta Mol Basis Dis 1870(7):167356. 2024 Oct. PMID: 39025375.