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Overview

Uniprot ID	Q9P219
Protein Name	Protein Daple
Gene Name	CCDC88C
Organism	Homo sapiens

Kla Sites from experimental identification

Position	Flanking peptide
122	KSMEEIKKVLLLVLG

Function

Required for activation of guanine nucleotide-binding proteins (G proteins) during non-canonical Wnt signaling (PubMed:26126266). Binds to ligand-activated Wnt receptor FZD7, displacing DVL1 from the FZD7 receptor and leading to inhibition of canonical Wnt signaling (PubMed:26126266). Acts as a non-receptor guanine nucleotide exchange factor by also binding to guanine nucleotide-binding protein G(i) alpha (Gi-alpha) subunits, leading to their activation (PubMed:26126266). Binding to Gi-alpha subunits displaces the beta and gamma subunits from the heterotrimeric G protein complex, triggering non-canonical Wnt responses such as activation of RAC1 and PI3K-AKT signaling (PubMed:26126266). Promotes apical constriction of cells via ARHGEF18 (PubMed:30948426)

Protein Sequence

10 MDVTVSELLE 20 LFLQSPLVTW 30 VKTFGPFGSG 40 SQDNLTMYMD 50 LVDGIFLNQI 60 MLQIDPRPTN 70 QRINKHVNND 80 VNLRIQNLTI 90 LVRNIKTYYQ 100 EVLQQLIVMN 110 LPNVLMIGRD 120 PLSGKSMEEI 130 KKVLLLVLGC 140 AVQCERKEEF 150 IERIKQLDIE 160 TQAGIVAHIQ 170 EVTHNQENVF 180 DLQWLELPDV 190 APEELEALSR 200 SMVLHLRRLI 210 DQRDECTELI 220 VDLTQERDYL 230 QAQHPPSPIK 240 SSSADSTPSP 250 TSSLSSEDKQ 260 HLAVELADTK 270 ARLRRVRQEL 280 EDKTEQLVDT 290 RHEVDQLVLE 300 LQKVKQENIQ 310 LAADARSARA 320 YRDELDSLRE 330 KANRVERLEL 340 ELTRCKEKLH 350 DVDFYKARME 360 ELREDNIILI 370 ETKAMLEEQL 380 TAARARGDKV 390 HELEKENLQL 400 KSKLHDLELD 410 RDTDKKRIEE 420 LLEENMVLEI 430 AQKQSMNESA 440 HLGWELEQLS 450 KNADLSDASR 460 KSFVFELNEC 470 ASSRILKLEK 480 ENQSLQSTIQ 490 GLRDASLVLE 500 ESGLKCGELE 510 KENHQLSKKI 520 EKLQTQLERE 530 KQSNQDLETL 540 SEELIREKEQ 550 LQSDMETLKA 560 DKARQIKDLE 570 QEKDHLNRAM 580 WSLRERSQVS 590 SEARMKDVEK 600 ENKALHQTVT 610 EANGKLSQLE 620 FEKRQLHRDL 630 EQAKEKGERA 640 EKLERELQRL 650 QEENGRLARK 660 VTSLETATEK 670 VEALEHESQG 680 LQLENRTLRK 690 SLDTLQNVSL 700 QLEGLERDNK 710 QLDAENLELR 720 RLVETMRFTS 730 TKLAQMEREN 740 QQLEREKEEL 750 RKNVDLLKAL 760 GKKSERLELS 770 YQSVSAENLR 780 LQQSLESSSH 790 KTQTLESELG 800 ELEAERQALR 810 RDLEALRLAN 820 AQLEGAEKDR 830 KALEQEVAQL 840 EKDKKLLEKE 850 AKRLWQQVEL 860 KDAVLDDSTA 870 KLSAVEKESR 880 ALDKELARCR 890 DAAGKLKELE 900 KDNRDLTKQV 910 TVHARTLTTL 920 REDLVLEKLK 930 SQQLSSELDK 940 LSQELEKVGL 950 NRELLLQEDD 960 SGSDTKYKIL 970 EGRNESALKT 980 TLAMKEEKIV 990 LLEAQMEEKA 1000 SLNRQLESEL 1010 QMLKKECETL 1020 RQNQGEGQHL 1030 QNSFKHPAGK 1040 TAASHQGKEA 1050 WGPGHKEATM 1060 ELLRVKDRAI 1070 ELERNNAALQ 1080 AEKQLLKEQL 1090 QHLETQNVTF 1100 SSQILTLQKQ 1110 SAFLQEHNTT 1120 LQTQTAKLQV 1130 ENSTLSSQSA 1140 ALTAQYTLLQ 1150 NHHTAKETEN 1160 ESLQRQQEQL 1170 TAAYEALLQD 1180 HEHLGTLHER 1190 QSAEYEALIR 1200 QHSCLKTLHR 1210 NLELEHKELG 1220 ERHGDMLKRK 1230 AELEEREKVL 1240 TTEREALQQE 1250 QRTNALAMGE 1260 NQRLRGELDR 1270 VNFLHHQLKG 1280 EYEELHAHTK 1290 ELKTSLNNAQ 1300 LELNRWQARF 1310 DELKEQHQTM 1320 DISLTKLDNH 1330 CELLSRLKGN 1340 LEEENHHLLS 1350 QIQLLSQQNQ 1360 MLLEQNMENK 1370 EQYHEEQKQY 1380 IDKLNALRRH 1390 KEKLEEKIMD 1400 QYKFYDPPPK 1410 KKNHWIGAKA 1420 LVKLIKPKKE 1430 GSRERLKSTV 1440 DSPPWQLESS 1450 DPASPAASQP 1460 LRSQAENPDT 1470 PALGSNCAEE 1480 RDAHNGSVGK 1490 GPGDLKPKRG 1500 SPHRGSLDRT 1510 DASTDLAMRS 1520 WPSELGSRTC 1530 STSATTTAPS 1540 NSTPIARHPG 1550 RTKGYNSDDN 1560 LCEPSLEFEV 1570 PNHRQYVSRP 1580 SSLESSRNTS 1590 SNSSPLNLKG 1600 SSEQLHGRSE 1610 SFSSEDLIPS 1620 RDLATLPREA 1630 STPGRNALGR 1640 HEYPLPRNGP 1650 LPQEGAQKRG 1660 TAPPYVGVRP 1670 CSASPSSEMV 1680 TLEEFLEESN 1690 RSSPTHDTPS 1700 CRDDLLSDYF 1710 RKASDPPAIG 1720 GQPGPPAKKE 1730 GAKMPTNFVA 1740 PTVKMAAPTS 1750 EGRPLKPGQY 1760 VKPNFRLTEA 1770 EAPPSVAPRQ 1780 AQPPQSLSLG 1790 RPRQAPVPPA 1800 SHAPASRSAS 1810 LSRAFSLASA 1820 DLLRASGPEA 1830 CKQESPQKLG 1840 APEALGGRET 1850 GSHTLQSPAP 1860 PSSHSLARER 1870 TPLVGKAGSS 1880 CQGPGPRSRP 1890 LDTRRFSLAP 1900 PKEERLAPLH 1910 QSATAPAIAT 1920 AGAGAAAAGS 1930 GSNSQLLHFS 1940 PAAAPAARTK 1950 PKAPPRSGEV 1960 ATITPVRAGL 1970 SLSEGDGVPG 1980 QGCSEGLPAK 1990 SPGRSPDLAP 2000 HLGRALEDCS 2010 RGSVSKSSPA 2020 SPEPGGDPQT VWYEYGCV

Gene Ontology

Classification	GO ID	Description
Cellular Component	GO:0043296	apical junction complex
Cellular Component	GO:0030054	cell junction
Cellular Component	GO:0005813	centrosome
Cellular Component	GO:0005737	cytoplasm
Molecular Function	GO:0051959	dynein light intermediate chain binding
Molecular Function	GO:0005109	frizzled binding
Molecular Function	GO:0001965	G-protein alpha-subunit binding
Molecular Function	GO:0005085	guanyl-nucleotide exchange factor activity
Molecular Function	GO:0042802	identical protein binding
Molecular Function	GO:0008017	microtubule binding
Molecular Function	GO:0030165	PDZ domain binding
Molecular Function	GO:0046983	protein dimerization activity
Biological Process	GO:0003383	apical constriction
Biological Process	GO:0031122	cytoplasmic microtubule organization
Biological Process	GO:0030705	cytoskeleton-dependent intracellular transport
Biological Process	GO:0090090	negative regulation of canonical Wnt signaling pathway
Biological Process	GO:0035567	non-canonical Wnt signaling pathway
Biological Process	GO:0046330	positive regulation of JNK cascade
Biological Process	GO:0031648	protein destabilization
Biological Process	GO:0007264	small GTPase-mediated signal transduction

Reference

[1] Guo X, Ren X, Yan C, Huang H. Quantitative Proteomics Reveals the Role of Lysine Lactylation in Lenalidomide-Resistance in Multiple Myeloma Cells.. ACS Chem Biol 20(7):1728-1738. 2025 Jul 18. PMID: 40590393.