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Overview

Uniprot IDQ9QYR9
Protein NameAcyl-coenzyme A thioesterase 2, mitochondrial
Gene NameAcot2
OrganismMus musculus

Kla Sites from experimental identification

Position Flanking peptide
263 LRSHPEVKGPGIGLL
344 LQSPLVDKKSFIPVE
345 QSPLVDKKSFIPVER
379 FYADEISKRLQAHGK
83 RSALRDEKGALFRAH

Function

Catalyzes the hydrolysis of acyl-CoAs into free fatty acids and coenzyme A (CoASH), regulating their respective intracellular levels (PubMed:25114170). Displays higher activity toward long chain acyl CoAs (C14-C20) (PubMed:25114170). The enzyme is involved in enhancing the hepatic fatty acid oxidation in mitochondria (PubMed:25114170)

Protein Sequence

10 MVASSFAVLR 20 ASRLCQQDWK 30 SWARLFVPPP 40 LSTGGRTTWA 50 RTNATLSVEP 60 EGRSCWDEPL 70 SIAVRGLAPE 80 QPVTLRSALR 90 DEKGALFRAH 100 ARYRADAGGE 110 LNLARAPALG 120 GSFSGLEPMG 130 LLWAMEPERP 140 LWRLIKRDVQ 150 TPFLVELEVL 160 DGHEPDGGQR 170 LAQAVHERHF 180 LAPGVRRVPV 190 REGRVRATLF 200 LPPEPGPFPG 210 IIDLFGVGGG 220 LLEYRASLLA 230 GKGFAVMALA 240 YYNYDDLPKS 250 IETMHMEYFE 260 EAVNYLRSHP 270 EVKGPGIGLL 280 GISKGGELGL 290 AMASFLKGIT 300 AAVVINGSVA 310 AVGNTISYKD 320 ETIPPVSLLR 330 NQVKMTKDGL 340 LDVVEALQSP 350 LVDKKSFIPV 360 ERSDTTFLFL 370 VGQDDHNWKS 380 EFYADEISKR 390 LQAHGKEKPQ 400 IICYPAAGHY 410 IEPPYFPLCS 420 AGMHLLVGAN 430 ITFGGEPRAH 440 AVAQVDAWQQ 450 LQTFFHKQLG SKS

Gene Ontology

Classification GO ID Description
Cellular Component GO:0005759 mitochondrial matrix
Cellular Component GO:0005739 mitochondrion
Molecular Function GO:0052689 carboxylic ester hydrolase activity
Molecular Function GO:0047617 fatty acyl-CoA hydrolase activity
Molecular Function GO:0052816 long-chain fatty acyl-CoA hydrolase activity
Biological Process GO:0006637 acyl-CoA metabolic process
Biological Process GO:0006631 fatty acid metabolic process
Biological Process GO:0001676 long-chain fatty acid metabolic process
Biological Process GO:0036116 long-chain fatty-acyl-CoA catabolic process
Biological Process GO:0001666 response to hypoxia
Biological Process GO:0000038 very long-chain fatty acid metabolic process

Reference

[1] Zhuo W, Zhang M, Tan J, Gao Y, Wang Y et al.. Lysine lactylation analysis of proteins in the heart of the Kawasaki disease mouse model.. Front Cell Dev Biol 13:1550220. 2025. PMID: 40114965.

[2] Wu D, Tang Y, Li X, Xiong S, Zhang Z et al.. Characterization of protein lactylation in healthy and ischemic mouse hearts.. Front Cardiovasc Med 12:1644886. 2025. PMID: 41089239.