Search Results

Overview

Uniprot IDQ9R0X4
Protein NameAcyl-coenzyme A thioesterase 9, mitochondrial
Gene NameAcot9
OrganismMus musculus

Kla Sites from experimental identification

Position Flanking peptide
102 TDPELRDKYVTVQNT
231 KEEEELFKQGELNKS
292 AVWMEDTKLKSLDIC
294 WMEDTKLKSLDICHP
432 MSTPVTLKKDYPVEP

Function

Mitochondrial acyl-CoA thioesterase. Catalyzes the hydrolysis of acyl-CoAs into free fatty acids and coenzyme A (CoA), regulating their respective intracellular levels (PubMed:10383425, PubMed:23864032). Shows a clear preference for hydrophobic short-, medium-, and long-chain saturated acyl-CoAs with some activity also with short-chain dicarboxylic CoA esters (PubMed:10383425, PubMed:23864032). Regulates both mitochondrial lipid and amino acid metabolism (PubMed:23864032, PubMed:31676684, PubMed:32498134)

Protein Sequence

10 MKRAAIRLWT 20 LNKGLLTHGR 30 GLSQGSQYKI 40 SEPLHIHQVR 50 DKLREIVGVS 60 TVWRDHVKAM 70 EERKLLHSFL 80 PKSQKVLPPR 90 KMRDSYIEVL 100 LPLGTDPELR 110 DKYVTVQNTV 120 RFGRILEDLD 130 SLGVLVCYMH 140 NHNHSTKMSP 150 LSIVTVLVDK 160 IDMCKHSLSP 170 EQDIKFTGHV 180 SWVGNTSMEV 190 KMKMFQLHND 200 EKYWPVLDAT 210 FVMVARDSEN 220 KGPAFVNPLI 230 PENKEEEELF 240 KQGELNKSRR 250 IAFSTSSLLK 260 VAPSSEERNI 270 IHELFLTTLD 280 PKTISFQSRI 290 LPPKAVWMED 300 TKLKSLDICH 310 PQERNVFNRI 320 FGGFLMRKAY 330 ELAWATACSF 340 GGSRPYVVTV 350 DDIMFQKPVE 360 VGSLLFLSSQ 370 VCFTQDNYIQ 380 VRVHSEVSSL 390 DSREHMTTNV 400 FHFTFMSEKE 410 VPLIFPKTYG 420 ESMLYLDGQR 430 HFKSMSTPVT LKKDYPVEP

Gene Ontology

Classification GO ID Description
Cellular Component GO:0005743 mitochondrial inner membrane
Cellular Component GO:0005759 mitochondrial matrix
Cellular Component GO:0005739 mitochondrion
Molecular Function GO:0003986 acetyl-CoA hydrolase activity
Molecular Function GO:0052689 carboxylic ester hydrolase activity
Molecular Function GO:0047617 fatty acyl-CoA hydrolase activity
Molecular Function GO:0052816 long-chain fatty acyl-CoA hydrolase activity
Biological Process GO:0006637 acyl-CoA metabolic process
Biological Process GO:0001676 long-chain fatty acid metabolic process
Biological Process GO:0046459 short-chain fatty acid metabolic process

Reference

[1] Zhuo W, Zhang M, Tan J, Gao Y, Wang Y et al.. Lysine lactylation analysis of proteins in the heart of the Kawasaki disease mouse model.. Front Cell Dev Biol 13:1550220. 2025. PMID: 40114965.

[2] Wu D, Tang Y, Li X, Xiong S, Zhang Z et al.. Characterization of protein lactylation in healthy and ischemic mouse hearts.. Front Cardiovasc Med 12:1644886. 2025. PMID: 41089239.