Overview
| Uniprot ID | Q9UBP0 |
|---|
| Protein Name | Spastin |
|---|
| Gene Name | SPAST |
|---|
| Organism | Homo sapiens |
|---|
Kla Sites from experimental identification
| Position |
Flanking peptide |
| 254 |
PRSKTVMKTGSAGLS |
Function
ATP-dependent microtubule severing protein that specifically recognizes and cuts microtubules that are polyglutamylated (PubMed:11809724, PubMed:15716377, PubMed:16219033, PubMed:17389232, PubMed:20530212, PubMed:22637577, PubMed:26875866). Preferentially recognizes and acts on microtubules decorated with short polyglutamate tails: severing activity increases as the number of glutamates per tubulin rises from one to eight, but decreases beyond this glutamylation threshold (PubMed:26875866). Severing activity is not dependent on tubulin acetylation or detyrosination (PubMed:26875866). Microtubule severing promotes reorganization of cellular microtubule arrays and the release of microtubules from the centrosome following nucleation. It is critical for the biogenesis and maintenance of complex microtubule arrays in axons, spindles and cilia. SPAST is involved in abscission step of cytokinesis and nuclear envelope reassembly during anaphase in cooperation with the ESCRT-III complex (PubMed:19000169, PubMed:21310966, PubMed:26040712). Recruited at the midbody, probably by IST1, and participates in membrane fission during abscission together with the ESCRT-III complex (PubMed:21310966). Recruited to the nuclear membrane by IST1 and mediates microtubule severing, promoting nuclear envelope sealing and mitotic spindle disassembly during late anaphase (PubMed:26040712). Required for membrane traffic from the endoplasmic reticulum (ER) to the Golgi and endosome recycling (PubMed:23897888). Recruited by IST1 to endosomes and regulates early endosomal tubulation and recycling by mediating microtubule severing (PubMed:23897888). Probably plays a role in axon growth and the formation of axonal branches (PubMed:15716377)
Protein Sequence
10
MNSPGGRGKK
20
KGSGGASNPV
30
PPRPPPPCLA
40
PAPPAAGPAP
50
PPESPHKRNL
60
YYFSYPLFVG
70
FALLRLVAFH
80
LGLLFVWLCQ
90
RFSRALMAAK
100
RSSGAAPAPA
110
SASAPAPVPG
120
GEAERVRVFH
130
KQAFEYISIA
140
LRIDEDEKAG
150
QKEQAVEWYK
160
KGIEELEKGI
170
AVIVTGQGEQ
180
CERARRLQAK
190
MMTNLVMAKD
200
RLQLLEKMQP
210
VLPFSKSQTD
220
VYNDSTNLAC
230
RNGHLQSESG
240
AVPKRKDPLT
250
HTSNSLPRSK
260
TVMKTGSAGL
270
SGHHRAPSYS
280
GLSMVSGVKQ
290
GSGPAPTTHK
300
GTPKTNRTNK
310
PSTPTTATRK
320
KKDLKNFRNV
330
DSNLANLIMN
340
EIVDNGTAVK
350
FDDIAGQDLA
360
KQALQEIVIL
370
PSLRPELFTG
380
LRAPARGLLL
390
FGPPGNGKTM
400
LAKAVAAESN
410
ATFFNISAAS
420
LTSKYVGEGE
430
KLVRALFAVA
440
RELQPSIIFI
450
DEVDSLLCER
460
REGEHDASRR
470
LKTEFLIEFD
480
GVQSAGDDRV
490
LVMGATNRPQ
500
ELDEAVLRRF
510
IKRVYVSLPN
520
EETRLLLLKN
530
LLCKQGSPLT
540
QKELAQLARM
550
TDGYSGSDLT
560
ALAKDAALGP
570
IRELKPEQVK
580
NMSASEMRNI
590
RLSDFTESLK
600
KIKRSVSPQT
610
LEAYIRWNKD
FGDTTV
Gene Ontology
| Classification |
GO ID |
Description |
| Cellular Component |
GO:0030424 |
axon |
| Cellular Component |
GO:1904115 |
axon cytoplasm |
| Cellular Component |
GO:0005813 |
centrosome |
| Cellular Component |
GO:0005737 |
cytoplasm |
| Cellular Component |
GO:0031410 |
cytoplasmic vesicle |
| Cellular Component |
GO:0005829 |
cytosol |
| Cellular Component |
GO:0005789 |
endoplasmic reticulum membrane |
| Cellular Component |
GO:0071782 |
endoplasmic reticulum tubular network |
| Cellular Component |
GO:0005768 |
endosome |
| Cellular Component |
GO:0070062 |
extracellular exosome |
| Cellular Component |
GO:0005811 |
lipid droplet |
| Cellular Component |
GO:0005874 |
microtubule |
| Cellular Component |
GO:0030496 |
midbody |
| Cellular Component |
GO:0031965 |
nuclear membrane |
| Cellular Component |
GO:0005654 |
nucleoplasm |
| Cellular Component |
GO:0005634 |
nucleus |
| Cellular Component |
GO:0048471 |
perinuclear region of cytoplasm |
| Cellular Component |
GO:0005819 |
spindle |
| Cellular Component |
GO:0000922 |
spindle pole |
| Molecular Function |
GO:0043014 |
alpha-tubulin binding |
| Molecular Function |
GO:0005524 |
ATP binding |
| Molecular Function |
GO:0016887 |
ATP hydrolysis activity |
| Molecular Function |
GO:0048487 |
beta-tubulin binding |
| Molecular Function |
GO:0008017 |
microtubule binding |
| Molecular Function |
GO:0008568 |
microtubule severing ATPase activity |
| Molecular Function |
GO:0044877 |
protein-containing complex binding |
| Biological Process |
GO:0008089 |
anterograde axonal transport |
| Biological Process |
GO:0019896 |
axonal transport of mitochondrion |
| Biological Process |
GO:0021955 |
central nervous system neuron axonogenesis |
| Biological Process |
GO:0032506 |
cytokinetic process |
| Biological Process |
GO:0061640 |
cytoskeleton-dependent cytokinesis |
| Biological Process |
GO:0006888 |
endoplasmic reticulum to Golgi vesicle-mediated transport |
| Biological Process |
GO:0010458 |
exit from mitosis |
| Biological Process |
GO:0090148 |
membrane fission |
| Biological Process |
GO:0001578 |
microtubule bundle formation |
| Biological Process |
GO:0051013 |
microtubule severing |
| Biological Process |
GO:0000281 |
mitotic cytokinesis |
| Biological Process |
GO:0007084 |
mitotic nuclear membrane reassembly |
| Biological Process |
GO:0051228 |
mitotic spindle disassembly |
| Biological Process |
GO:0031468 |
nuclear membrane reassembly |
| Biological Process |
GO:0032467 |
positive regulation of cytokinesis |
| Biological Process |
GO:0031117 |
positive regulation of microtubule depolymerization |
| Biological Process |
GO:0034214 |
protein hexamerization |
| Biological Process |
GO:0051260 |
protein homooligomerization |
Reference
PMID: N/A