Overview
| Uniprot ID | Q9UIS9 |
|---|
| Protein Name | Methyl-CpG-binding domain protein 1 |
|---|
| Gene Name | MBD1 |
|---|
| Organism | Homo sapiens |
|---|
Kla Sites from experimental identification
| Position |
Flanking peptide |
| 84 |
HPVAVASKKRKKPSR |
| 85 |
PVAVASKKRKKPSRP |
Function
Transcriptional repressor that binds CpG islands in promoters where the DNA is methylated at position 5 of cytosine within CpG dinucleotides. Binding is abolished by the presence of 7-mG that is produced by DNA damage by methylmethanesulfonate (MMS). Acts as transcriptional repressor and plays a role in gene silencing by recruiting ATF7IP, which in turn recruits factors such as the histone methyltransferase SETDB1. Probably forms a complex with SETDB1 and ATF7IP that represses transcription and couples DNA methylation and histone 'Lys-9' trimethylation. Isoform 1 and isoform 2 can also repress transcription from unmethylated promoters
Protein Sequence
10
MAEDWLDCPA
20
LGPGWKRREV
30
FRKSGATCGR
40
SDTYYQSPTG
50
DRIRSKVELT
60
RYLGPACDLT
70
LFDFKQGILC
80
YPAPKAHPVA
90
VASKKRKKPS
100
RPAKTRKRQV
110
GPQSGEVRKE
120
APRDETKADT
130
DTAPASFPAP
140
GCCENCGISF
150
SGDGTQRQRL
160
KTLCKDCRAQ
170
RIAFNREQRM
180
FKRVGCGECA
190
ACQVTEDCGA
200
CSTCLLQLPH
210
DVASGLFCKC
220
ERRRCLRIVE
230
RSRGCGVCRG
240
CQTQEDCGHC
250
PICLRPPRPG
260
LRRQWKCVQR
270
RCLRGKHARR
280
KGGCDSKMAA
290
RRRPGAQPLP
300
PPPPSQSPEP
310
TEPHPRALAP
320
SPPAEFIYYC
330
VDEDELQPYT
340
NRRQNRKCGA
350
CAACLRRMDC
360
GRCDFCCDKP
370
KFGGSNQKRQ
380
KCRWRQCLQF
390
AMKRLLPSVW
400
SESEDGAGSP
410
PPYRRRKRPS
420
SARRHHLGPT
430
LKPTLATRTA
440
QPDHTQAPTK
450
QEAGGGFVLP
460
PPGTDLVFLR
470
EGASSPVQVP
480
GPVAASTEAL
490
LQEAQCSGLS
500
WVVALPQVKQ
510
EKADTQDEWT
520
PGTAVLTSPV
530
LVPGCPSKAV
540
DPGLPSVKQE
550
PPDPEEDKEE
560
NKDDSASKLA
570
PEEEAGGAGT
580
PVITEIFSLG
590
GTRFRDTAVW
600
LPRSKDLKKP
GARKQ
Gene Ontology
| Classification |
GO ID |
Description |
| Cellular Component |
GO:0005694 |
chromosome |
| Cellular Component |
GO:0016363 |
nuclear matrix |
| Cellular Component |
GO:0016607 |
nuclear speck |
| Cellular Component |
GO:0005654 |
nucleoplasm |
| Cellular Component |
GO:0005634 |
nucleus |
| Molecular Function |
GO:0003677 |
DNA binding |
| Molecular Function |
GO:0010385 |
double-stranded methylated DNA binding |
| Molecular Function |
GO:0008327 |
methyl-CpG binding |
| Molecular Function |
GO:0045322 |
unmethylated CpG binding |
| Molecular Function |
GO:0008270 |
zinc ion binding |
| Biological Process |
GO:0006346 |
DNA methylation-dependent constitutive heterochromatin formation |
| Biological Process |
GO:0045892 |
negative regulation of DNA-templated transcription |
| Biological Process |
GO:0000122 |
negative regulation of transcription by RNA polymerase II |
| Biological Process |
GO:0006366 |
transcription by RNA polymerase II |
Reference
[1] Wu Q, Li Z, Gong T, Zheng X, Zhou X et al.. Porphyromonas gingivalis infection induces lysine lactylation reprogramming in human umbilical vein endothelial cells.. Front Cell Infect Microbiol 16:1706727. 2026. PMID: 41696360.