Overview
| Uniprot ID | Q9UKG1 |
|---|
| Protein Name | DCC-interacting protein 13-alpha |
|---|
| Gene Name | APPL1 |
|---|
| Organism | Homo sapiens |
|---|
Kla Sites from experimental identification
| Position |
Flanking peptide |
| 657 |
QKELNKQKQIEKDLE |
Function
Multifunctional adapter protein that binds to various membrane receptors, nuclear factors and signaling proteins to regulate many processes, such as cell proliferation, immune response, endosomal trafficking and cell metabolism (PubMed:10490823, PubMed:15016378, PubMed:19661063, PubMed:26073777, PubMed:26583432). Regulates signaling pathway leading to cell proliferation through interaction with RAB5A and subunits of the NuRD/MeCP1 complex (PubMed:15016378). Functions as a positive regulator of innate immune response via activation of AKT1 signaling pathway by forming a complex with APPL1 and PIK3R1 (By similarity). Inhibits Fc-gamma receptor-mediated phagocytosis through PI3K/Akt signaling in macrophages (By similarity). Regulates TLR4 signaling in activated macrophages (By similarity). Involved in trafficking of the TGFBR1 from the endosomes to the nucleus via microtubules in a TRAF6-dependent manner (PubMed:26583432). Plays a role in cell metabolism by regulating adiponecting and insulin signaling pathways (PubMed:19661063, PubMed:24879834, PubMed:26073777). Required for fibroblast migration through HGF cell signaling (By similarity). Positive regulator of beta-catenin/TCF-dependent transcription through direct interaction with RUVBL2/reptin resulting in the relief of RUVBL2-mediated repression of beta-catenin/TCF target genes by modulating the interactions within the beta-catenin-reptin-HDAC complex (PubMed:19433865)
Protein Sequence
10
MPGIDKLPIE
20
ETLEDSPQTR
30
SLLGVFEEDA
40
TAISNYMNQL
50
YQAMHRIYDA
60
QNELSAATHL
70
TSKLLKEYEK
80
QRFPLGGDDE
90
VMSSTLQQFS
100
KVIDELSSCH
110
AVLSTQLADA
120
MMFPITQFKE
130
RDLKEILTLK
140
EVFQIASNDH
150
DAAINRYSRL
160
SKKRENDKVK
170
YEVTEDVYTS
180
RKKQHQTMMH
190
YFCALNTLQY
200
KKKIALLEPL
210
LGYMQAQISF
220
FKMGSENLNE
230
QLEEFLANIG
240
TSVQNVRREM
250
DSDIETMQQT
260
IEDLEVASDP
270
LYVPDPDPTK
280
FPVNRNLTRK
290
AGYLNARNKT
300
GLVSSTWDRQ
310
FYFTQGGNLM
320
SQARGDVAGG
330
LAMDIDNCSV
340
MAVDCEDRRY
350
CFQITSFDGK
360
KSSILQAESK
370
KDHEEWICTI
380
NNISKQIYLS
390
ENPEETAARV
400
NQSALEAVTP
410
SPSFQQRHES
420
LRPAAGQSRP
430
PTARTSSSGS
440
LGSESTNLAA
450
LSLDSLVAPD
460
TPIQFDIISP
470
VCEDQPGQAK
480
AFGQGGRRTN
490
PFGESGGSTK
500
SETEDSILHQ
510
LFIVRFLGSM
520
EVKSDDHPDV
530
VYETMRQILA
540
ARAIHNIFRM
550
TESHLLVTCD
560
CLKLIDPQTQ
570
VTRLTFPLPC
580
VVLYATHQEN
590
KRLFGFVLRT
600
SSGRSESNLS
610
SVCYIFESNN
620
EGEKICDSVG
630
LAKQIALHAE
640
LDRRASEKQK
650
EIERVKEKQQ
660
KELNKQKQIE
670
KDLEEQSRLI
680
AASSRPNQAS
690
SEGQFVVLSS
700
SQSEESDLGE
GGKKRESEA
Gene Ontology
| Classification |
GO ID |
Description |
| Cellular Component |
GO:0005737 |
cytoplasm |
| Cellular Component |
GO:0031410 |
cytoplasmic vesicle |
| Cellular Component |
GO:0005829 |
cytosol |
| Cellular Component |
GO:0005769 |
early endosome |
| Cellular Component |
GO:0031901 |
early endosome membrane |
| Cellular Component |
GO:0032009 |
early phagosome |
| Cellular Component |
GO:0005768 |
endosome |
| Cellular Component |
GO:0010008 |
endosome membrane |
| Cellular Component |
GO:0070062 |
extracellular exosome |
| Cellular Component |
GO:0098978 |
glutamatergic synapse |
| Cellular Component |
GO:0097708 |
intracellular vesicle |
| Cellular Component |
GO:0044354 |
macropinosome |
| Cellular Component |
GO:0016020 |
membrane |
| Cellular Component |
GO:0005634 |
nucleus |
| Cellular Component |
GO:0005886 |
plasma membrane |
| Cellular Component |
GO:0001726 |
ruffle |
| Cellular Component |
GO:0012506 |
vesicle membrane |
| Molecular Function |
GO:0048487 |
beta-tubulin binding |
| Molecular Function |
GO:0042802 |
identical protein binding |
| Molecular Function |
GO:0035091 |
phosphatidylinositol binding |
| Molecular Function |
GO:0001786 |
phosphatidylserine binding |
| Molecular Function |
GO:0042803 |
protein homodimerization activity |
| Molecular Function |
GO:0043422 |
protein kinase B binding |
| Molecular Function |
GO:0044877 |
protein-containing complex binding |
| Biological Process |
GO:0033211 |
adiponectin-activated signaling pathway |
| Biological Process |
GO:0035729 |
cellular response to hepatocyte growth factor stimulus |
| Biological Process |
GO:0008286 |
insulin receptor signaling pathway |
| Biological Process |
GO:0099558 |
maintenance of synapse structure |
| Biological Process |
GO:1905450 |
negative regulation of Fc-gamma receptor signaling pathway involved in phagocytosis |
| Biological Process |
GO:1900017 |
positive regulation of cytokine production involved in inflammatory response |
| Biological Process |
GO:0046326 |
positive regulation of D-glucose import across plasma membrane |
| Biological Process |
GO:1905303 |
positive regulation of macropinocytosis |
| Biological Process |
GO:0048023 |
positive regulation of melanin biosynthetic process |
| Biological Process |
GO:0006606 |
protein import into nucleus |
| Biological Process |
GO:0046324 |
regulation of D-glucose import across plasma membrane |
| Biological Process |
GO:0010762 |
regulation of fibroblast migration |
| Biological Process |
GO:2000045 |
regulation of G1/S transition of mitotic cell cycle |
| Biological Process |
GO:0045088 |
regulation of innate immune response |
| Biological Process |
GO:1903076 |
regulation of protein localization to plasma membrane |
| Biological Process |
GO:0034143 |
regulation of toll-like receptor 4 signaling pathway |
| Biological Process |
GO:0007165 |
signal transduction |
| Biological Process |
GO:0023052 |
signaling |
| Biological Process |
GO:0007179 |
transforming growth factor beta receptor signaling pathway |
Reference
[1] He C, Zhang J, Bai X, Lu C, Zhang K. Lysine lactylation-based insight to understanding the characterization of cervical cancer.. Biochim Biophys Acta Mol Basis Dis 1870(7):167356. 2024 Oct. PMID: 39025375.