Search Results

Overview

Uniprot IDQ9UKK9
Protein NameADP-sugar pyrophosphatase
Gene NameNUDT5
OrganismHomo sapiens

Kla Sites from experimental identification

Position Flanking peptide
218 PFEVPFLKF******
33 GKWVKLEKTTYMDPT
42 TYMDPTGKTRTWESV
50 TRTWESVKRTTRKEQ
55 SVKRTTRKEQTADGV

Function

Enzyme that can either act as an ADP-sugar pyrophosphatase in absence of diphosphate or catalyze the synthesis of ATP in presence of diphosphate (PubMed:27257257). In absence of diphosphate, hydrolyzes with similar activities various modified nucleoside diphosphates such as ADP-ribose, ADP-mannose, ADP-glucose, 8-oxo-GDP and 8-oxo-dGDP (PubMed:10567213, PubMed:10722730, PubMed:17052728, PubMed:12717453, PubMed:19699693, PubMed:21389046, PubMed:38635563). Can also hydrolyze other nucleotide sugars with low activity (PubMed:12717453, PubMed:19699693, PubMed:21389046). In presence of diphosphate, mediates the synthesis of ATP in the nucleus by catalyzing the conversion of ADP-ribose to ATP and ribose 5-phosphate. Nuclear ATP synthesis takes place when dephosphorylated at Thr-45 (PubMed:27257257). Nuclear ATP generation is required for extensive chromatin remodeling events that are energy-consuming (PubMed:27257257). In vitro, it has decapping activity on 11-mer RNA capped with uridine diphosphate N-acetylglucosamine (UDP-GlcNAc) by hydrolyzing the pyrophosphate bridge of the sugar cap (PubMed:38716860). Does not play a role in U8 snoRNA decapping (By similarity). Binds U8 snoRNA (By similarity)

Protein Sequence

10 MESQEPTESS 20 QNGKQYIISE 30 ELISEGKWVK 40 LEKTTYMDPT 50 GKTRTWESVK 60 RTTRKEQTAD 70 GVAVIPVLQR 80 TLHYECIVLV 90 KQFRPPMGGY 100 CIEFPAGLID 110 DGETPEAAAL 120 RELEEETGYK 130 GDIAECSPAV 140 CMDPGLSNCT 150 IHIVTVTING 160 DDAENARPKP 170 KPGDGEFVEV 180 ISLPKNDLLQ 190 RLDALVAEEH 200 LTVDARVYSY 210 ALALKHANAK PFEVPFLKF

Gene Ontology

Classification GO ID Description
Cellular Component GO:0005829 cytosol
Cellular Component GO:0070062 extracellular exosome
Cellular Component GO:0005634 nucleus
Molecular Function GO:0044715 8-oxo-dGDP phosphatase activity
Molecular Function GO:0047631 ADP-ribose diphosphatase activity
Molecular Function GO:0019144 ADP-sugar diphosphatase activity
Molecular Function GO:0042802 identical protein binding
Molecular Function GO:0000287 magnesium ion binding
Molecular Function GO:0016779 nucleotidyltransferase activity
Molecular Function GO:0042803 protein homodimerization activity
Molecular Function GO:0030515 snoRNA binding
Biological Process GO:1990966 ATP generation from poly-ADP-D-ribose
Biological Process GO:0006338 chromatin remodeling
Biological Process GO:0019303 D-ribose catabolic process
Biological Process GO:0055086 nucleobase-containing small molecule metabolic process
Biological Process GO:0006753 nucleoside phosphate metabolic process
Biological Process GO:0009117 nucleotide metabolic process
Biological Process GO:0009191 ribonucleoside diphosphate catabolic process
Biological Process GO:0019693 ribose phosphate metabolic process

Reference

[1] Yang Z, Yan C, Ma J, Peng P, Ren X et al.. Lactylome analysis suggests lactylation-dependent mechanisms of metabolic adaptation in hepatocellular carcinoma.. Nat Metab 5(1):61-79. 2023 Jan. PMID: 36593272.

[2] Hong H, Chen X, Wang H, Gu X, Yuan Y et al.. Global profiling of protein lysine lactylation and potential target modified protein analysis in hepatocellular carcinoma.. Proteomics 23(9):e2200432. 2023 May. PMID: 36625413.

[3] Wu Q, Li Z, Gong T, Zheng X, Zhou X et al.. Porphyromonas gingivalis infection induces lysine lactylation reprogramming in human umbilical vein endothelial cells.. Front Cell Infect Microbiol 16:1706727. 2026. PMID: 41696360.