Overview
| Uniprot ID | Q9UKT4 |
|---|
| Protein Name | F-box only protein 5 |
|---|
| Gene Name | FBXO5 |
|---|
| Organism | Homo sapiens |
|---|
Kla Sites from experimental identification
| Position |
Flanking peptide |
| 289 |
KKILEDDKGAFQLYS |
| 344 |
LKKDAQTKLSNQGDQ |
Function
Regulator of APC activity during mitotic and meiotic cell cycle (PubMed:16921029, PubMed:17234884, PubMed:17485488, PubMed:17875940, PubMed:23708001, PubMed:23708605). During mitotic cell cycle plays a role as both substrate and inhibitor of APC-FZR1 complex (PubMed:16921029, PubMed:17234884, PubMed:17485488, PubMed:17875940, PubMed:23708001, PubMed:23708605, PubMed:29875408). During G1 phase, plays a role as substrate of APC-FZR1 complex E3 ligase (PubMed:29875408). Then switches as an inhibitor of APC-FZR1 complex during S and G2 leading to cell-cycle commitment (PubMed:29875408). As APC inhibitor, prevents the degradation of APC substrates at multiple levels: by interacting with APC and blocking access of APC substrates to the D-box coreceptor, formed by FZR1 and ANAPC10; by suppressing ubiquitin ligation and chain elongation by APC by preventing the UBE2C and UBE2S activities (PubMed:16921029, PubMed:23708001, PubMed:23708605). Plays a role in genome integrity preservation by coordinating DNA replication with mitosis through APC inhibition in interphase to stabilize CCNA2 and GMNN in order to promote mitosis and prevent rereplication and DNA damage-induced cellular senescence (PubMed:17234884, PubMed:17485488, PubMed:17875940). During oocyte maturation, plays a role in meiosis through inactivation of APC-FZR1 complex. Inhibits APC through RPS6KA2 interaction that increases FBXO5 affiniy for CDC20 leading to the metaphase arrest of the second meiotic division before fertilization (By similarity). Controls entry into the first meiotic division through inactivation of APC-FZR1 complex (By similarity). Promotes migration and osteogenic differentiation of mesenchymal stem cells (PubMed:29850565)
Protein Sequence
10
MSRRPCSCAL
20
RPPRCSCSAS
30
PSAVTAAGRP
40
RPSDSCKEES
50
STLSVKMKCD
60
FNCNHVHSGL
70
KLVKPDDIGR
80
LVSYTPAYLE
90
GSCKDCIKDY
100
ERLSCIGSPI
110
VSPRIVQLET
120
ESKRLHNKEN
130
QHVQQTLNST
140
NEIEALETSR
150
LYEDSGYSSF
160
SLQSGLSEHE
170
EGSLLEENFG
180
DSLQSCLLQI
190
QSPDQYPNKN
200
LLPVLHFEKV
210
VCSTLKKNAK
220
RNPKVDREML
230
KEIIARGNFR
240
LQNIIGRKMG
250
LECVDILSEL
260
FRRGLRHVLA
270
TILAQLSDMD
280
LINVSKVSTT
290
WKKILEDDKG
300
AFQLYSKAIQ
310
RVTENNNKFS
320
PHASTREYVM
330
FRTPLASVQK
340
SAAQTSLKKD
350
AQTKLSNQGD
360
QKGSTYSRHN
370
EFSEVAKTLK
380
KNESLKACIR
390
CNSPAKYDCY
400
LQRATCKREG
410
CGFDYCTKCL
420
CNYHTTKDCS
430
DGKLLKASCK
440
IGPLPGTKKS
KKNLRRL
Gene Ontology
| Classification |
GO ID |
Description |
| Cellular Component |
GO:0005737 |
cytoplasm |
| Cellular Component |
GO:0005829 |
cytosol |
| Cellular Component |
GO:0072687 |
meiotic spindle |
| Cellular Component |
GO:0005654 |
nucleoplasm |
| Cellular Component |
GO:0005634 |
nucleus |
| Cellular Component |
GO:0005819 |
spindle |
| Molecular Function |
GO:0010997 |
anaphase-promoting complex binding |
| Molecular Function |
GO:0140678 |
molecular function inhibitor activity |
| Molecular Function |
GO:0019901 |
protein kinase binding |
| Molecular Function |
GO:1990948 |
ubiquitin ligase inhibitor activity |
| Molecular Function |
GO:0008270 |
zinc ion binding |
| Biological Process |
GO:0051301 |
cell division |
| Biological Process |
GO:0006974 |
DNA damage response |
| Biological Process |
GO:2000773 |
negative regulation of cellular senescence |
| Biological Process |
GO:0032876 |
negative regulation of DNA endoreduplication |
| Biological Process |
GO:0045835 |
negative regulation of meiotic nuclear division |
| Biological Process |
GO:0045841 |
negative regulation of mitotic metaphase/anaphase transition |
| Biological Process |
GO:1904667 |
negative regulation of ubiquitin protein ligase activity |
| Biological Process |
GO:0051444 |
negative regulation of ubiquitin-protein transferase activity |
| Biological Process |
GO:0070169 |
positive regulation of biomineral tissue development |
| Biological Process |
GO:0008284 |
positive regulation of cell population proliferation |
| Biological Process |
GO:0010971 |
positive regulation of G2/M transition of mitotic cell cycle |
| Biological Process |
GO:1905322 |
positive regulation of mesenchymal stem cell migration |
| Biological Process |
GO:0045669 |
positive regulation of osteoblast differentiation |
| Biological Process |
GO:0016567 |
protein ubiquitination |
| Biological Process |
GO:0006275 |
regulation of DNA replication |
| Biological Process |
GO:0007346 |
regulation of mitotic cell cycle |
| Biological Process |
GO:0007088 |
regulation of mitotic nuclear division |
| Biological Process |
GO:0031146 |
SCF-dependent proteasomal ubiquitin-dependent protein catabolic process |
Reference
[1] Yang Z, Yan C, Ma J, Peng P, Ren X et al.. Lactylome analysis suggests lactylation-dependent mechanisms of metabolic adaptation in hepatocellular carcinoma.. Nat Metab 5(1):61-79. 2023 Jan. PMID: 36593272.