Search Results

Overview

Uniprot IDQ9ULX6
Protein NameA-kinase anchor protein 8-like
Gene NameAKAP8L
OrganismHomo sapiens

Kla Sites from experimental identification

Position Flanking peptide
264 KQMRRTWKTWTTADF
279 RTKKKKRKQGGSPDE
369 KRKLQAGKKSQDKQK
370 RKLQAGKKSQDKQKK
374 AGKKSQDKQKKRQRD
533 ARSILNNKLISKKLE

Function

Could play a role in constitutive transport element (CTE)-mediated gene expression by association with DHX9. Increases CTE-dependent nuclear unspliced mRNA export (PubMed:10748171, PubMed:11402034). Proposed to target PRKACA to the nucleus but does not seem to be implicated in the binding of regulatory subunit II of PKA (PubMed:10761695, PubMed:11884601). May be involved in nuclear envelope breakdown and chromatin condensation. May be involved in anchoring nuclear membranes to chromatin in interphase and in releasing membranes from chromating at mitosis (PubMed:11034899). May regulate the initiation phase of DNA replication when associated with TMPO isoform Beta (PubMed:12538639). Required for cell cycle G2/M transition and histone deacetylation during mitosis. In mitotic cells recruits HDAC3 to the vicinity of chromatin leading to deacetylation and subsequent phosphorylation at 'Ser-10' of histone H3; in this function seems to act redundantly with AKAP8 (PubMed:16980585). May be involved in regulation of pre-mRNA splicing (PubMed:17594903)

Protein Sequence

10 MSYTGFVQGS 20 ETTLQSTYSD 30 TSAQPTCDYG 40 YGTWNSGTNR 50 GYEGYGYGYG 60 YGQDNTTNYG 70 YGMATSHSWE 80 MPSSDTNANT 90 SASGSASADS 100 VLSRINQRLD 110 MVPHLETDMM 120 QGGVYGSGGE 130 RYDSYESCDS 140 RAVLSERDLY 150 RSGYDYSELD 160 PEMEMAYEGQ 170 YDAYRDQFRM 180 RGNDTFGPRA 190 QGWARDARSG 200 RPMASGYGRM 210 WEDPMGARGQ 220 CMSGASRLPS 230 LFSQNIIPEY 240 GMFQGMRGGG 250 AFPGGSRFGF 260 GFGNGMKQMR 270 RTWKTWTTAD 280 FRTKKKKRKQ 290 GGSPDEPDSK 300 ATRTDCSDNS 310 DSDNDEGTEG 320 EATEGLEGTE 330 AVEKGSRVDG 340 EDEEGKEDGR 350 EEGKEDPEKG 360 ALTTQDENGQ 370 TKRKLQAGKK 380 SQDKQKKRQR 390 DRMVERIQFV 400 CSLCKYRTFY 410 EDEMASHLDS 420 KFHKEHFKYV 430 GTKLPKQTAD 440 FLQEYVTNKT 450 KKTEELRKTV 460 EDLDGLIQQI 470 YRDQDLTQEI 480 AMEHFVKKVE 490 AAHCAACDLF 500 IPMQFGIIQK 510 HLKTMDHNRN 520 RRLMMEQSKK 530 SSLMVARSIL 540 NNKLISKKLE 550 RYLKGENPFT 560 DSPEEEKEQE 570 EAEGGALDEG 580 AQGEAAGISE 590 GAEGVPAQPP 600 VPPEPAPGAV 610 SPPPPPPPEE 620 EEEGAVPLLG 630 GALQRQIRGI 640 PGLDVEDDEE GGGGAP

Gene Ontology

Classification GO ID Description
Cellular Component GO:0000785 chromatin
Cellular Component GO:0016363 nuclear matrix
Cellular Component GO:0016607 nuclear speck
Cellular Component GO:0005654 nucleoplasm
Cellular Component GO:0005634 nucleus
Cellular Component GO:0016605 PML body
Cellular Component GO:1990904 ribonucleoprotein complex
Molecular Function GO:0017151 DEAD/H-box RNA helicase binding
Molecular Function GO:0003677 DNA binding
Molecular Function GO:0042826 histone deacetylase binding
Molecular Function GO:0005521 lamin binding
Molecular Function GO:0034237 protein kinase A regulatory subunit binding
Molecular Function GO:0003723 RNA binding
Molecular Function GO:0008270 zinc ion binding
Biological Process GO:0044839 cell cycle G2/M phase transition
Biological Process GO:0006351 DNA-templated transcription
Biological Process GO:0007076 mitotic chromosome condensation
Biological Process GO:0006397 mRNA processing
Biological Process GO:0051081 nuclear membrane disassembly
Biological Process GO:0045944 positive regulation of transcription by RNA polymerase II
Biological Process GO:0010793 regulation of mRNA export from nucleus
Biological Process GO:0008380 RNA splicing
Cellular Component GO:0005737 cytoplasm

Reference

[1] Yang D, Yin J, Shan L, Yi X, Zhang W et al.. Identification of lysine-lactylated substrates in gastric cancer cells.. iScience 25(7):104630. 2022 Jul 15. PMID: 35800753.

[2] Yang Z, Yan C, Ma J, Peng P, Ren X et al.. Lactylome analysis suggests lactylation-dependent mechanisms of metabolic adaptation in hepatocellular carcinoma.. Nat Metab 5(1):61-79. 2023 Jan. PMID: 36593272.

[3] Shi CM, Wang QC, Li XL, Yang YH, Tang XY et al.. Global Profiling of Protein Lactylation in Human Hippocampi.. Proteomics Clin Appl 19(2):e202400061. 2025 Mar. PMID: 39610256.

[4] Chao L, Xu Y, Yang Y, Ao X, Liang J. Identification of lactylation-related biomarkers for diagnosis, prognosis, and treatment responsiveness in triple-negative breast cancer.. World J Surg Oncol 24(1):77. 2026 Jan 22. PMID: 41566505.