Overview
| Uniprot ID | Q9UNU6 |
|---|
| Protein Name | 7-alpha-hydroxycholest-4-en-3-one 12-alpha-hydroxylase |
|---|
| Gene Name | CYP8B1 |
|---|
| Organism | Homo sapiens |
|---|
Kla Sites from experimental identification
| Position |
Flanking peptide |
| 104 |
LDFGQYAKKLVLKVF |
Function
Sterol 12-alpha-hydroxylase involved in primary bile acid biosynthesis by catalyzing the 12alpha-hydroxylation of key intermediates including 7alpha-hydroxycholest-4-en-3-one, 3alpha,7alpha-dihydroxy-5beta-cholestan-26-oate and chenodeoxycholate along the classic pathway (PubMed:10051404, PubMed:30465713, PubMed:39214664). May also hydroxylate 5beta-cholestane-3alpha,7alpha-diol (By similarity). Functions as a monooxygenase, inserting one atom of molecular oxygen into substrates while reducing the second to water, using electrons supplied by NADPH via cytochrome P450 reductase (CPR) (By similarity). Controls biliary balance of cholic acid and chenodeoxycholic acid, thereby regulating intestinal absorption of dietary lipids (By similarity)
Protein Sequence
10
MVLWGPVLGA
20
LLVVIAGYLC
30
LPGMLRQRRP
40
WEPPLDKGTV
50
PWLGHAMAFR
60
KNMFEFLKRM
70
RTKHGDVFTV
80
QLGGQYFTFV
90
MDPLSFGSIL
100
KDTQRKLDFG
110
QYAKKLVLKV
120
FGYRSVQGDH
130
EMIHSASTKH
140
LRGDGLKDLN
150
ETMLDSLSFV
160
MLTSKGWSLD
170
ASCWHEDSLF
180
RFCYYILFTA
190
GYLSLFGYTK
200
DKEQDLLQAG
210
ELFMEFRKFD
220
LLFPRFVYSL
230
LWPREWLEVG
240
RLQRLFHKML
250
SVSHSQEKEG
260
ISNWLGNMLQ
270
FLREQGVPSA
280
MQDKFNFMML
290
WASQGNTGPT
300
SFWALLYLLK
310
HPEAIRAVRE
320
EATQVLGEAR
330
LETKQSFAFK
340
LGALQHTPVL
350
DSVVEETLRL
360
RAAPTLLRLV
370
HEDYTLKMSS
380
GQEYLFRHGD
390
ILALFPYLSV
400
HMDPDIHPEP
410
TVFKYDRFLN
420
PNGSRKVDFF
430
KTGKKIHHYT
440
MPWGSGVSIC
450
PGRFFALSEV
460
KLFILLMVTH
470
FDLELVDPDT
480
PLPHVDPQRW
490
GFGTMQPSHD
500
VRFRYRLHPT
E
Gene Ontology
| Classification |
GO ID |
Description |
| Cellular Component |
GO:0005789 |
endoplasmic reticulum membrane |
| Molecular Function |
GO:0020037 |
heme binding |
| Molecular Function |
GO:0005506 |
iron ion binding |
| Molecular Function |
GO:0019825 |
oxygen binding |
| Molecular Function |
GO:0008397 |
sterol 12-alpha-hydroxylase activity |
| Biological Process |
GO:0006699 |
bile acid biosynthetic process |
| Biological Process |
GO:0045797 |
positive regulation of intestinal cholesterol absorption |
| Biological Process |
GO:0070723 |
response to cholesterol |
| Biological Process |
GO:0031667 |
response to nutrient levels |
| Biological Process |
GO:0006694 |
steroid biosynthetic process |
| Biological Process |
GO:0016125 |
sterol metabolic process |
Reference
[1] Yang Z, Yan C, Ma J, Peng P, Ren X et al.. Lactylome analysis suggests lactylation-dependent mechanisms of metabolic adaptation in hepatocellular carcinoma.. Nat Metab 5(1):61-79. 2023 Jan. PMID: 36593272.