Search Results

Overview

Uniprot IDQ9UNZ2
Protein NameNSFL1 cofactor p47
Gene NameNSFL1C
OrganismHomo sapiens

Kla Sites from experimental identification

Position Flanking peptide
112 QIVGPPRKKSPNELV
113 IVGPPRKKSPNELVD
124 ELVDDLFKGAKEHGA
127 DDLFKGAKEHGAVAV
139 VAVERVTKSPGETSK
146 KSPGETSKPRPFAGG
172 SAYVAGEKRQHSSQD
247 DEDFVKPKGAFKAFT
251 VKPKGAFKAFTGEGQ
259 AFTGEGQKLGSTAPQ

Function

Reduces the ATPase activity of VCP (By similarity). Necessary for the fragmentation of Golgi stacks during mitosis and for VCP-mediated reassembly of Golgi stacks after mitosis (By similarity). May play a role in VCP-mediated formation of transitional endoplasmic reticulum (tER) (By similarity). Inhibits the activity of CTSL (in vitro) (PubMed:15498563). Together with UBXN2B/p37, regulates the centrosomal levels of kinase AURKA/Aurora A during mitotic progression by promoting AURKA removal from centrosomes in prophase (PubMed:23649807). Also, regulates spindle orientation during mitosis (PubMed:23649807)

Protein Sequence

10 MAAERQEALR 20 EFVAVTGAEE 30 DRARFFLESA 40 GWDLQIALAS 50 FYEDGGDEDI 60 VTISQATPSS 70 VSRGTAPSDN 80 RVTSFRDLIH 90 DQDEDEEEEE 100 GQRFYAGGSE 110 RSGQQIVGPP 120 RKKSPNELVD 130 DLFKGAKEHG 140 AVAVERVTKS 150 PGETSKPRPF 160 AGGGYRLGAA 170 PEEESAYVAG 180 EKRQHSSQDV 190 HVVLKLWKSG 200 FSLDNGELRS 210 YQDPSNAQFL 220 ESIRRGEVPA 230 ELRRLAHGGQ 240 VNLDMEDHRD 250 EDFVKPKGAF 260 KAFTGEGQKL 270 GSTAPQVLST 280 SSPAQQAENE 290 AKASSSILID 300 ESEPTTNIQI 310 RLADGGRLVQ 320 KFNHSHRISD 330 IRLFIVDARP 340 AMAATSFILM 350 TTFPNKELAD 360 ESQTLKEANL 370 LNAVIVQRLT

Gene Ontology

Classification GO ID Description
Cellular Component GO:0005813 centrosome
Cellular Component GO:0005694 chromosome
Cellular Component GO:0005737 cytoplasm
Cellular Component GO:0005829 cytosol
Cellular Component GO:0005795 Golgi stack
Cellular Component GO:0005634 nucleus
Cellular Component GO:1990730 VCP-NSFL1C complex
Molecular Function GO:0008289 lipid binding
Molecular Function GO:0043130 ubiquitin binding
Biological Process GO:0000045 autophagosome assembly
Biological Process GO:0000132 establishment of mitotic spindle orientation
Biological Process GO:0007030 Golgi organization
Biological Process GO:0061025 membrane fusion
Biological Process GO:1904780 negative regulation of protein localization to centrosome
Biological Process GO:0031468 nuclear membrane reassembly
Biological Process GO:0046604 positive regulation of mitotic centrosome separation
Biological Process GO:0043161 proteasome-mediated ubiquitin-dependent protein catabolic process
Biological Process GO:0006511 ubiquitin-dependent protein catabolic process

Reference

[1] Yang D, Yin J, Shan L, Yi X, Zhang W et al.. Identification of lysine-lactylated substrates in gastric cancer cells.. iScience 25(7):104630. 2022 Jul 15. PMID: 35800753.

[2] Yang Z, Yan C, Ma J, Peng P, Ren X et al.. Lactylome analysis suggests lactylation-dependent mechanisms of metabolic adaptation in hepatocellular carcinoma.. Nat Metab 5(1):61-79. 2023 Jan. PMID: 36593272.

[3] Hong H, Chen X, Wang H, Gu X, Yuan Y et al.. Global profiling of protein lysine lactylation and potential target modified protein analysis in hepatocellular carcinoma.. Proteomics 23(9):e2200432. 2023 May. PMID: 36625413.

[4] Cheng Z, Huang H, Li M, Chen Y. Proteomic analysis identifies PFKP lactylation in SW480 colon cancer cells.. iScience 27(1):108645. 2024 Jan 19. PMID: 38155775.

[5] He C, Zhang J, Bai X, Lu C, Zhang K. Lysine lactylation-based insight to understanding the characterization of cervical cancer.. Biochim Biophys Acta Mol Basis Dis 1870(7):167356. 2024 Oct. PMID: 39025375.

[6] Bao Q, Wan N, He Z, Cao J, Yuan W et al.. Subcellular Proteomic Mapping of Lysine Lactylation.. J Am Soc Mass Spectrom 35(12):3221-3232. 2024 Dec 4. PMID: 39569522.

[7] Guo X, Ren X, Yan C, Huang H. Quantitative Proteomics Reveals the Role of Lysine Lactylation in Lenalidomide-Resistance in Multiple Myeloma Cells.. ACS Chem Biol 20(7):1728-1738. 2025 Jul 18. PMID: 40590393.

[8] He J, Lai T, Zhou Z, Yang H, Lei Z et al.. Multiomics profiling reveals the involvement of protein lactylation in nonhomologous end joining pathway conferring radioresistance in lung adenocarcinoma cell.. Sci Rep 15(1):24651. 2025 Jul 9. PMID: 40634431.

[9] Wu Q, Li Z, Gong T, Zheng X, Zhou X et al.. Porphyromonas gingivalis infection induces lysine lactylation reprogramming in human umbilical vein endothelial cells.. Front Cell Infect Microbiol 16:1706727. 2026. PMID: 41696360.