Search Results

Overview

Uniprot IDQ9UPN6
Protein NameSR-related and CTD-associated factor 8
Gene NameSCAF8
OrganismHomo sapiens

Kla Sites from experimental identification

Position Flanking peptide
999 DNVTNPEKRIPLGND

Function

Anti-terminator protein required to prevent early mRNA termination during transcription (PubMed:31104839). Together with SCAF4, acts by suppressing the use of early, alternative poly(A) sites, thereby preventing the accumulation of non-functional truncated proteins (PubMed:31104839). Mechanistically, associates with the phosphorylated C-terminal heptapeptide repeat domain (CTD) of the largest RNA polymerase II subunit (POLR2A), and subsequently binds nascent RNA upstream of early polyadenylation sites to prevent premature mRNA transcript cleavage and polyadenylation (PubMed:31104839). Independently of SCAF4, also acts as a positive regulator of transcript elongation (PubMed:31104839)

Protein Sequence

10 MEAVKTFNSE 20 LYSLNDYKPP 30 ISKAKMTQIT 40 KAAIKAIKFY 50 KHVVQSVEKF 60 IQKCKPEYKV 70 PGLYVIDSIV 80 RQSRHQFGQE 90 KDVFAPRFSN 100 NIISTFQNLY 110 RCPGDDKSKI 120 VRVLNLWQKN 130 NVFKSEIIQP 140 LLDMAAGIPP 150 PVVTPVLAST 160 TTAMSNTPGT 170 PVTPVTPANV 180 VQGLPDPWVS 190 QITNTDTLAA 200 VAQILQSPQG 210 QQLQQLIQTL 220 QIQQQKPQPS 230 ILQALDAGLV 240 VQLQALTAQL 250 TAAAAAANTL 260 TPLEQGVSFN 270 KKLMDRFDFG 280 EDSEHSEEPK 290 KEIPASQLSH 300 VSESVNNSIF 310 HQIAEQLQQQ 320 NLEHLRQQLL 330 EQQQPQKATP 340 QDSQEGTFGS 350 EHSASPSQGS 360 SQQHFLEPEV 370 NLDDSIDIQQ 380 QDMDIDEGQD 390 GVEEEVFEQE 400 AKKVAVRSRS 410 RTHSRSRSRS 420 PRKRRSRSRS 430 GSRKRKHRKR 440 SRSRSRERKR 450 KSSRSYSSER 460 RAREREKERQ 470 KKGLPPIRSK 480 TLSVCSTTLW 490 VGQVDKKATQ 500 QDLTNLFEEF 510 GQIESINMIP 520 PRGCAYVCMV 530 HRQDAFRALQ 540 KLSSGSYKIG 550 SKVIKIAWAL 560 NKGVKTEYKQ 570 FWDVDLGVTY 580 IPWEKVKVDD 590 LEGFAEGGMI 600 DQETVNTEWE 610 TVKSSEPVKE 620 TVQTTQSPTP 630 VEKETVVTTQ 640 AEVFPPPVAM 650 LQIPVAPAVP 660 TVSLVPPAFP 670 VSMPVPPPGF 680 SPIPPPPFLR 690 ASFNPSQPPP 700 GFMPPPVPPP 710 VVPPPTIPPV 720 VPTSLVQPSL 730 SMTPETVKDV 740 GFGSLVIPGG 750 SVASNLATSA 760 LPAGNVFNAP 770 TKQAEPEEKV 780 PHLIDHQISS 790 GENTRSVIPN 800 DISSNAAILG 810 GQPPNVTSNS 820 GILGVQRPNV 830 SSNSEILGVR 840 PSNVSSSSGI 850 IAAQPPNILN 860 NSGILGIQPP 870 SVSNSSGLLG 880 VLPPNIPNNS 890 GLVGVQPPNV 900 PNTPGLLGTQ 910 PPAGPQNLPP 920 LSIPNQRMPT 930 MPMLDIRPGL 940 IPQAPGPRFP 950 LIQPGIPPQR 960 GIPPPSVLDS 970 ALHPPPRGPF 980 PPGDIFSQPE 990 RPFLAPGRQS 1000 VDNVTNPEKR 1010 IPLGNDNIQQ 1020 EGDRDYRFPP 1030 IETRESISRP 1040 PPVDVRDVVG 1050 RPIDPREGPG 1060 RPPLDGRDHF 1070 GRPPVDIREN 1080 LVRPGIDHLG 1090 RRDHFGFNPE 1100 KPWGHRGDFD 1110 EREHRVLPVY 1120 GGPKGLHEER 1130 GRFRSGNYRF 1140 DPRSGPWNRG 1150 FGQEVHRDFD 1160 DRRRPWERQR 1170 DRDDRDFDFC 1180 REMNGNRLGR 1190 DRIQNTWVPP 1200 PHARVFDYFE 1210 GATSQRKGDN 1220 VPQVNGENTE 1230 RHAQPPPIPV 1240 QNDPELYEKL 1250 TSSNEINKEK 1260 SDTVADIESE 1270 PVVESTETEG T

Gene Ontology

Classification GO ID Description
Cellular Component GO:0005737 cytoplasm
Cellular Component GO:0005849 mRNA cleavage factor complex
Cellular Component GO:0016363 nuclear matrix
Cellular Component GO:0005654 nucleoplasm
Cellular Component GO:0005634 nucleus
Molecular Function GO:0003729 mRNA binding
Molecular Function GO:0003723 RNA binding
Molecular Function GO:0043175 RNA polymerase core enzyme binding
Molecular Function GO:1990269 RNA polymerase II C-terminal domain phosphoserine binding
Molecular Function GO:0000993 RNA polymerase II complex binding
Biological Process GO:2000805 negative regulation of termination of RNA polymerase II transcription, poly(A)-coupled
Biological Process GO:0032786 positive regulation of DNA-templated transcription, elongation
Biological Process GO:0006369 termination of RNA polymerase II transcription

Reference

PMID: N/A