Overview
| Uniprot ID | Q9UQ88 |
|---|
| Protein Name | Cyclin-dependent kinase 11A |
|---|
| Gene Name | CDK11A |
|---|
| Organism | Homo sapiens |
|---|
Kla Sites from experimental identification
| Position |
Flanking peptide |
| 20 |
LDEILQEKKRRKEQE |
| 21 |
DEILQEKKRRKEQEE |
| 213 |
MREDYSDKVKASHWS |
| 412 |
ELKQELPKYLPALQG |
Function
Cyclin-dependent protein kinase that acts as a regulator of transcription and pre-mRNA splicing (PubMed:12501247, PubMed:32367068, PubMed:36104565, PubMed:40858114). Acts as a key regulator of pre-mRNA splicing by mediating phosphorylation of SF3B1, enabling the association between SF3B1 and U5 and U6 snRNAs in the activated spliceosome, thereby promoting spliceosome assembly (PubMed:36104565, PubMed:38059508). Also acts as a regulator of transcription by phosphorylating 'Ser-2' of the CTD (C-terminal domain) of the large subunit of RNA polymerase II (RNAP II) POLR2A (PubMed:32367068, PubMed:40858114). Involved in replication-dependent transcription of histone genes: binds to histone genes and phosphorylates POLR2A at 'Ser-2' of the CTD to specifically control transcriptional elongation of histones and recruitment of 3'-end processing factors (PubMed:32367068). Part of a transcription checkpoint upstream of CDK9, which regulates promoter-proximal pausing by RNA polymerase II, a transcription halt following transcription initiation, but prior to elongation (PubMed:40858114). Probably regulates promoter-proximal pausing by mediating phosphorylation of POLR2A at 'Ser-2' of the CTD (PubMed:40858114)
Protein Sequence
10
MGDEKDSWKV
20
KTLDEILQEK
30
KRRKEQEEKA
40
EIKRLKNSDD
50
RDSKRDSLEE
60
GELRDHCMEI
70
TIRNSPYRRE
80
DSMEDRGEED
90
DSLAIKPPQQ
100
MSRKEKVHHR
110
KDEKRKEKWK
120
HARVKEREHE
130
RRKRHREEQD
140
KARREWERQK
150
RREMAREHSR
160
RERDRLEQLE
170
RKRERERKMR
180
EQQKEQREQK
190
ERERRAEERR
200
KEREARREVS
210
AHHRTMREDY
220
SDKVKASHWS
230
RSPPRPPRER
240
FELGDGRKPG
250
EARPAPAQKP
260
AQLKEEKMEE
270
RDLLSDLQDI
280
SDSERKTSSA
290
ESSSAESGSG
300
SEEEEEEEEE
310
EEEEGSTSEE
320
SEEEEEEEEE
330
EEEETGSNSE
340
EASEQSAEEV
350
SEEEMSEDEE
360
RENENHLLVV
370
PESRFDRDSG
380
ESEEAEEEVG
390
EGTPQSSALT
400
EGDYVPDSPA
410
LLPIELKQEL
420
PKYLPALQGC
430
RSVEEFQCLN
440
RIEEGTYGVV
450
YRAKDKKTDE
460
IVALKRLKME
470
KEKEGFPITS
480
LREINTILKA
490
QHPNIVTVRE
500
IVVGSNMDKI
510
YIVMNYVEHD
520
LKSLMETMKQ
530
PFLPGEVKTL
540
MIQLLRGVKH
550
LHDNWILHRD
560
LKTSNLLLSH
570
AGILKVGDFG
580
LAREYGSPLK
590
AYTPVVVTQW
600
YRAPELLLGA
610
KEYSTAVDMW
620
SVGCIFGELL
630
TQKPLFPGNS
640
EIDQINKVFK
650
ELGTPSEKIW
660
PGYSELPVVK
670
KMTFSEHPYN
680
NLRKRFGALL
690
SDQGFDLMNK
700
FLTYFPGRRI
710
SAEDGLKHEY
720
FRETPLPIDP
730
SMFPTWPAKS
740
EQQRVKRGTS
750
PRPPEGGLGY
760
SQLGDDDLKE
770
TGFHLTTTNQ
780
GASAAGPGFS
LKF
Gene Ontology
| Classification |
GO ID |
Description |
| Cellular Component |
GO:0005694 |
chromosome |
| Cellular Component |
GO:0000775 |
chromosome, centromeric region |
| Cellular Component |
GO:0180038 |
cyclin L-CDK11 complex |
| Cellular Component |
GO:0000307 |
cyclin-dependent protein kinase holoenzyme complex |
| Cellular Component |
GO:0005829 |
cytosol |
| Cellular Component |
GO:0016607 |
nuclear speck |
| Cellular Component |
GO:0005634 |
nucleus |
| Molecular Function |
GO:0005524 |
ATP binding |
| Molecular Function |
GO:0004693 |
cyclin-dependent protein serine/threonine kinase activity |
| Molecular Function |
GO:0004672 |
protein kinase activity |
| Molecular Function |
GO:0106310 |
protein serine kinase activity |
| Molecular Function |
GO:0004674 |
protein serine/threonine kinase activity |
| Molecular Function |
GO:0140834 |
RNA polymerase II CTD heptapeptide repeat S2 kinase activity |
| Molecular Function |
GO:0140836 |
RNA polymerase II CTD heptapeptide repeat S5 kinase activity |
| Biological Process |
GO:0006915 |
apoptotic process |
| Biological Process |
GO:0000278 |
mitotic cell cycle |
| Biological Process |
GO:0006397 |
mRNA processing |
| Biological Process |
GO:0006468 |
protein phosphorylation |
| Biological Process |
GO:0042981 |
regulation of apoptotic process |
| Biological Process |
GO:0051726 |
regulation of cell cycle |
| Biological Process |
GO:0001558 |
regulation of cell growth |
| Biological Process |
GO:0046605 |
regulation of centrosome cycle |
| Biological Process |
GO:0006355 |
regulation of DNA-templated transcription |
| Biological Process |
GO:0050684 |
regulation of mRNA processing |
| Biological Process |
GO:0048024 |
regulation of mRNA splicing, via spliceosome |
| Biological Process |
GO:0043484 |
regulation of RNA splicing |
| Biological Process |
GO:0001111 |
RNA polymerase II promoter clearance |
| Biological Process |
GO:0008380 |
RNA splicing |
Reference
[1] Yang D, Yin J, Shan L, Yi X, Zhang W et al.. Identification of lysine-lactylated substrates in gastric cancer cells.. iScience 25(7):104630. 2022 Jul 15. PMID: 35800753.