Search Results

Overview

Uniprot IDQ9Y223
Protein NameBifunctional UDP-N-acetylglucosamine 2-epimerase/N-acetylmannosamine kinase
Gene NameGNE
OrganismHomo sapiens

Kla Sites from experimental identification

Position Flanking peptide
195 KLLSAKNKDYMSIIR

Function

Bifunctional enzyme that possesses both UDP-N-acetylglucosamine 2-epimerase and N-acetylmannosamine kinase activities, and serves as the initiator of the biosynthetic pathway leading to the production of N-acetylneuraminic acid (NeuAc), a critical precursor in the synthesis of sialic acids. By catalyzing this pivotal and rate-limiting step in sialic acid biosynthesis, this enzyme assumes a pivotal role in governing the regulation of cell surface sialylation, playing a role in embryonic angiogenesis (PubMed:10334995, PubMed:11326336, PubMed:14707127, PubMed:16503651, PubMed:2808337, PubMed:38237079). Sialic acids represent a category of negatively charged sugars that reside on the surface of cells as terminal components of glycoconjugates and mediate important functions in various cellular processes, including cell adhesion, signal transduction, and cellular recognition (PubMed:10334995, PubMed:14707127)

Protein Sequence

10 MEKNGNNRKL 20 RVCVATCNRA 30 DYSKLAPIMF 40 GIKTEPEFFE 50 LDVVVLGSHL 60 IDDYGNTYRM 70 IEQDDFDINT 80 RLHTIVRGED 90 EAAMVESVGL 100 ALVKLPDVLN 110 RLKPDIMIVH 120 GDRFDALALA 130 TSAALMNIRI 140 LHIEGGEVSG 150 TIDDSIRHAI 160 TKLAHYHVCC 170 TRSAEQHLIS 180 MCEDHDRILL 190 AGCPSYDKLL 200 SAKNKDYMSI 210 IRMWLGDDVK 220 SKDYIVALQH 230 PVTTDIKHSI 240 KMFELTLDAL 250 ISFNKRTLVL 260 FPNIDAGSKE 270 MVRVMRKKGI 280 EHHPNFRAVK 290 HVPFDQFIQL 300 VAHAGCMIGN 310 SSCGVREVGA 320 FGTPVINLGT 330 RQIGRETGEN 340 VLHVRDADTQ 350 DKILQALHLQ 360 FGKQYPCSKI 370 YGDGNAVPRI 380 LKFLKSIDLQ 390 EPLQKKFCFP 400 PVKENISQDI 410 DHILETLSAL 420 AVDLGGTNLR 430 VAIVSMKGEI 440 VKKYTQFNPK 450 TYEERINLIL 460 QMCVEAAAEA 470 VKLNCRILGV 480 GISTGGRVNP 490 REGIVLHSTK 500 LIQEWNSVDL 510 RTPLSDTLHL 520 PVWVDNDGNC 530 AALAERKFGQ 540 GKGLENFVTL 550 ITGTGIGGGI 560 IHQHELIHGS 570 SFCAAELGHL 580 VVSLDGPDCS 590 CGSHGCIEAY 600 ASGMALQREA 610 KKLHDEDLLL 620 VEGMSVPKDE 630 AVGALHLIQA 640 AKLGNAKAQS 650 ILRTAGTALG 660 LGVVNILHTM 670 NPSLVILSGV 680 LASHYIHIVK 690 DVIRQQALSS 700 VQDVDVVVSD 710 LVDPALLGAA 720 SMVLDYTTRR IY

Gene Ontology

Classification GO ID Description
Cellular Component GO:0005829 cytosol
Molecular Function GO:0005524 ATP binding
Molecular Function GO:0004553 hydrolase activity, hydrolyzing O-glycosyl compounds
Molecular Function GO:0046872 metal ion binding
Molecular Function GO:0009384 N-acylmannosamine kinase activity
Molecular Function GO:0008761 UDP-N-acetylglucosamine 2-epimerase activity
Biological Process GO:0006055 CMP-N-acetylneuraminate biosynthetic process
Biological Process GO:0006045 N-acetylglucosamine biosynthetic process
Biological Process GO:0046380 N-acetylneuraminate biosynthetic process
Biological Process GO:0006047 UDP-N-acetylglucosamine metabolic process

Reference

[1] He J, Lai T, Zhou Z, Yang H, Lei Z et al.. Multiomics profiling reveals the involvement of protein lactylation in nonhomologous end joining pathway conferring radioresistance in lung adenocarcinoma cell.. Sci Rep 15(1):24651. 2025 Jul 9. PMID: 40634431.