Overview
| Uniprot ID | Q9Y253 |
|---|
| Protein Name | DNA polymerase eta |
|---|
| Gene Name | POLH |
|---|
| Organism | Homo sapiens |
|---|
Kla Sites from experimental identification
| Position |
Flanking peptide |
| 682 |
SAVSHQGKRNPKSPL |
Function
DNA polymerase specifically involved in the DNA repair by translesion synthesis (TLS) (PubMed:10385124, PubMed:11743006, PubMed:16357261, PubMed:20388628, PubMed:24449906, PubMed:24553286, PubMed:38212351). Due to low processivity on both damaged and normal DNA, cooperates with the heterotetrameric (REV3L, REV7, POLD2 and POLD3) POLZ complex for complete bypass of DNA lesions. Inserts one or 2 nucleotide(s) opposite the lesion, the primer is further extended by the tetrameric POLZ complex. In the case of 1,2-intrastrand d(GpG)-cisplatin cross-link, inserts dCTP opposite the 3' guanine (PubMed:24449906). Particularly important for the repair of UV-induced pyrimidine dimers (PubMed:10385124, PubMed:11743006). Although inserts the correct base, may cause base transitions and transversions depending upon the context. May play a role in hypermutation at immunoglobulin genes (PubMed:11376341, PubMed:14734526). Forms a Schiff base with 5'-deoxyribose phosphate at abasic sites, but does not have any lyase activity, preventing the release of the 5'-deoxyribose phosphate (5'-dRP) residue. This covalent trapping of the enzyme by the 5'-dRP residue inhibits its DNA synthetic activity during base excision repair, thereby avoiding high incidence of mutagenesis (PubMed:14630940). Targets POLI to replication foci (PubMed:12606586)
Protein Sequence
10
MATGQDRVVA
20
LVDMDCFFVQ
30
VEQRQNPHLR
40
NKPCAVVQYK
50
SWKGGGIIAV
60
SYEARAFGVT
70
RSMWADDAKK
80
LCPDLLLAQV
90
RESRGKANLT
100
KYREASVEVM
110
EIMSRFAVIE
120
RASIDEAYVD
130
LTSAVQERLQ
140
KLQGQPISAD
150
LLPSTYIEGL
160
PQGPTTAEET
170
VQKEGMRKQG
180
LFQWLDSLQI
190
DNLTSPDLQL
200
TVGAVIVEEM
210
RAAIERETGF
220
QCSAGISHNK
230
VLAKLACGLN
240
KPNRQTLVSH
250
GSVPQLFSQM
260
PIRKIRSLGG
270
KLGASVIEIL
280
GIEYMGELTQ
290
FTESQLQSHF
300
GEKNGSWLYA
310
MCRGIEHDPV
320
KPRQLPKTIG
330
CSKNFPGKTA
340
LATREQVQWW
350
LLQLAQELEE
360
RLTKDRNDND
370
RVATQLVVSI
380
RVQGDKRLSS
390
LRRCCALTRY
400
DAHKMSHDAF
410
TVIKNCNTSG
420
IQTEWSPPLT
430
MLFLCATKFS
440
ASAPSSSTDI
450
TSFLSSDPSS
460
LPKVPVTSSE
470
AKTQGSGPAV
480
TATKKATTSL
490
ESFFQKAAER
500
QKVKEASLSS
510
LTAPTQAPMS
520
NSPSKPSLPF
530
QTSQSTGTEP
540
FFKQKSLLLK
550
QKQLNNSSVS
560
SPQQNPWSNC
570
KALPNSLPTE
580
YPGCVPVCEG
590
VSKLEESSKA
600
TPAEMDLAHN
610
SQSMHASSAS
620
KSVLEVTQKA
630
TPNPSLLAAE
640
DQVPCEKCGS
650
LVPVWDMPEH
660
MDYHFALELQ
670
KSFLQPHSSN
680
PQVVSAVSHQ
690
GKRNPKSPLA
700
CTNKRPRPEG
710
MQTLESFFKP
LTH
Gene Ontology
| Classification |
GO ID |
Description |
| Cellular Component |
GO:0005654 |
nucleoplasm |
| Cellular Component |
GO:0005634 |
nucleus |
| Cellular Component |
GO:0005657 |
replication fork |
| Cellular Component |
GO:0035861 |
site of double-strand break |
| Molecular Function |
GO:0003684 |
damaged DNA binding |
| Molecular Function |
GO:0003887 |
DNA-directed DNA polymerase activity |
| Molecular Function |
GO:0008270 |
zinc ion binding |
| Biological Process |
GO:0006281 |
DNA repair |
| Biological Process |
GO:0000731 |
DNA synthesis involved in DNA repair |
| Biological Process |
GO:0070987 |
error-free translesion synthesis |
| Biological Process |
GO:0042276 |
error-prone translesion synthesis |
| Biological Process |
GO:0006282 |
regulation of DNA repair |
| Biological Process |
GO:0009314 |
response to radiation |
| Biological Process |
GO:0010225 |
response to UV-C |
| Biological Process |
GO:0019985 |
translesion synthesis |
Reference
PMID: N/A