Search Results

Overview

Uniprot ID	Q9Y295
Protein Name	Developmentally-regulated GTP-binding protein 1
Gene Name	DRG1
Organism	Homo sapiens

Kla Sites from experimental identification

Position	Flanking peptide
46	RRELITPKGGGGGGP

Function

Catalyzes the conversion of GTP to GDP through hydrolysis of the gamma-phosphate bond in GTP (PubMed:23711155, PubMed:29915238, PubMed:37179472). Appears to have an intrinsic GTPase activity that is stimulated by ZC3H15/DFRP1 binding likely by increasing the affinity for the potassium ions (PubMed:23711155). When hydroxylated at C-3 of 'Lys-22' by JMJD7, may bind to RNA and play a role in translation (PubMed:19819225, PubMed:29915238). Binds to microtubules and promotes microtubule polymerization and stability that are required for mitotic spindle assembly during prophase to anaphase transition. GTPase activity is not necessary for these microtubule-related functions (PubMed:28855639)

Protein Sequence

10 MSSTLAKIAE 20 IEAEMARTQK 30 NKATAHHLGL 40 LKARLAKLRR 50 ELITPKGGGG 60 GGPGEGFDVA 70 KTGDARIGFV 80 GFPSVGKSTL 90 LSNLAGVYSE 100 VAAYEFTTLT 110 TVPGVIRYKG 120 AKIQLLDLPG 130 IIEGAKDGKG 140 RGRQVIAVAR 150 TCNLILIVLD 160 VLKPLGHKKI 170 IENELEGFGI 180 RLNSKPPNIG 190 FKKKDKGGIN 200 LTATCPQSEL 210 DAETVKSILA 220 EYKIHNADVT 230 LRSDATADDL 240 IDVVEGNRVY 250 IPCIYVLNKI 260 DQISIEELDI 270 IYKVPHCVPI 280 SAHHRWNFDD 290 LLEKIWDYLK 300 LVRIYTKPKG 310 QLPDYTSPVV 320 LPYSRTTVED 330 FCMKIHKNLI 340 KEFKYALVWG 350 LSVKHNPQKV 360 GKDHTLEDED VIQIVKK

Gene Ontology

Classification	GO ID	Description
Cellular Component	GO:0005737	cytoplasm
Cellular Component	GO:0005829	cytosol
Cellular Component	GO:0016020	membrane
Cellular Component	GO:0016604	nuclear body
Cellular Component	GO:0005654	nucleoplasm
Cellular Component	GO:0005634	nucleus
Molecular Function	GO:0005525	GTP binding
Molecular Function	GO:0003924	GTPase activity
Molecular Function	GO:0008017	microtubule binding
Molecular Function	GO:0030955	potassium ion binding
Biological Process	GO:0002181	cytoplasmic translation
Biological Process	GO:0031116	positive regulation of microtubule polymerization
Biological Process	GO:1901673	regulation of mitotic spindle assembly

Reference

[1] Yang Z, Yan C, Ma J, Peng P, Ren X et al.. Lactylome analysis suggests lactylation-dependent mechanisms of metabolic adaptation in hepatocellular carcinoma.. Nat Metab 5(1):61-79. 2023 Jan. PMID: 36593272.

[2] Guo X, Ren X, Yan C, Huang H. Quantitative Proteomics Reveals the Role of Lysine Lactylation in Lenalidomide-Resistance in Multiple Myeloma Cells.. ACS Chem Biol 20(7):1728-1738. 2025 Jul 18. PMID: 40590393.