Search Results

Overview

Uniprot IDQ9Y2I7
Protein Name1-phosphatidylinositol 3-phosphate 5-kinase
Gene NamePIKFYVE
OrganismHomo sapiens

Kla Sites from experimental identification

Position Flanking peptide
2045 IRTFTWDKKLEMVVK
2046 RTFTWDKKLEMVVKS

Function

Dual specificity kinase implicated in myriad essential cellular processes such as maintenance of endomembrane homeostasis, and endocytic-vacuolar pathway, lysosomal trafficking, nuclear transport, stress- or hormone-induced signaling and cell cycle progression (PubMed:23086417). The PI(3,5)P2 regulatory complex regulates both the synthesis and turnover of phosphatidylinositol 3,5-bisphosphate (PtdIns(3,5)P2) (PubMed:17556371, PubMed:41639454). Sole enzyme to catalyze the phosphorylation of phosphatidylinositol 3-phosphate on the fifth hydroxyl of the myo-inositol ring, to form (PtdIns(3,5)P2) (PubMed:17556371, PubMed:41639454). Also catalyzes the phosphorylation of phosphatidylinositol on the fifth hydroxyl of the myo-inositol ring, to form phosphatidylinositol 5-phosphate (PtdIns(5)P) (PubMed:22621786). Has serine-protein kinase activity and is able to autophosphorylate and transphosphorylate. Autophosphorylation inhibits its own phosphatidylinositol 3-phosphate 5-kinase activity, stimulates FIG4 lipid phosphatase activity and down-regulates lipid product formation (PubMed:33098764). Involved in key endosome operations such as fission and fusion in the course of endosomal cargo transport (PubMed:22621786). Required for the maturation of early into late endosomes, phagosomes and lysosomes (PubMed:30612035). Regulates vacuole maturation and nutrient recovery following engulfment of macromolecules, initiates the redistribution of accumulated lysosomal contents back into the endosome network (PubMed:27623384). Critical regulator of the morphology, degradative activity, and protein turnover of the endolysosomal system in macrophages and platelets (By similarity). In neutrophils, critical to perform chemotaxis, generate ROS, and undertake phagosome fusion with lysosomes (PubMed:28779020). Plays a key role in the processing and presentation of antigens by major histocompatibility complex class II (MHC class II) mediated by CTSS (PubMed:30612035). Regulates melanosome biogenesis by controlling the delivery of proteins from the endosomal compartment to the melanosome (PubMed:29584722). Essential for systemic glucose homeostasis, mediates insulin-induced signals for endosome/actin remodeling in the course of GLUT4 translocation/glucose uptake activation (By similarity). Synthesis if (PtdIns(3,5)P2) is required for the cGAS-STING pathway (PubMed:41639454). Supports microtubule-based endosome-to-trans-Golgi network cargo transport, through association with SPAG9 and RABEPK (By similarity). Mediates EGFR trafficking to the nucleus (PubMed:17909029)

Protein Sequence

10 MATDDKTSPT 20 LDSANDLPRS 30 PTSPSHLTHF 40 KPLTPDQDEP 50 PFKSAYSSFV 60 NLFRFNKERA 70 EGGQGEQQPL 80 SGSWTSPQLP 90 SRTQSVRSPT 100 PYKKQLNEEL 110 QRRSSALDTR 120 RKAEPTFGGH 130 DPRTAVQLRS 140 LSTVLKRLKE 150 IMEGKSQDSD 160 LKQYWMPDSQ 170 CKECYDCSEK 180 FTTFRRRHHC 190 RLCGQIFCSR 200 CCNQEIPGKF 210 MGYTGDLRAC 220 TYCRKIALSY 230 AHSTDSNSIG 240 EDLNALSDSA 250 CSVSVLDPSE 260 PRTPVGSRKA 270 SRNIFLEDDL 280 AWQSLIHPDS 290 SNTPLSTRLV 300 SVQEDAGKSP 310 ARNRSASITN 320 LSLDRSGSPM 330 VPSYETSVSP 340 QANRTYVRTE 350 TTEDERKILL 360 DSVQLKDLWK 370 KICHHSSGME 380 FQDHRYWLRT 390 HPNCIVGKEL 400 VNWLIRNGHI 410 ATRAQAIAIG 420 QAMVDGRWLD 430 CVSHHDQLFR 440 DEYALYRPLQ 450 STEFSETPSP 460 DSDSVNSVEG 470 HSEPSWFKDI 480 KFDDSDTEQI 490 AEEGDDNLAN 500 SASPSKRTSV 510 SSFQSTVDSD 520 SAASISLNVE 530 LDNVNFHIKK 540 PSKYPHVPPH 550 PADQKEYLIS 560 DTGGQQLSIS 570 DAFIKESLFN 580 RRVEEKSKEL 590 PFTPLGWHHN 600 NLELLREENG 610 EKQAMERLLS 620 ANHNHMMALL 630 QQLLHSDSLS 640 SSWRDIIVSL 650 VCQVVQTVRP 660 DVKNQDDDMD 670 IRQFVHIKKI 680 PGGKKFDSVV 690 VNGFVCTKNI 700 AHKKMSSCIK 710 NPKILLLKCS 720 IEYLYREETK 730 FTCIDPIVLQ 740 EREFLKNYVQ 750 RIVDVRPTLV 760 LVEKTVSRIA 770 QDMLLEHGIT 780 LVINVKSQVL 790 ERISRMTQGD 800 LVMSMDQLLT 810 KPHLGTCHKF 820 YMQIFQLPNE 830 QTKTLMFFEG 840 CPQHLGCTIK 850 LRGGSDYELA 860 RVKEILIFMI 870 CVAYHSQLEI 880 SFLMDEFAMP 890 PTLMQNPSFH 900 SLIEGRGHEG 910 AVQEQYGGGS 920 IPWDPDIPPE 930 SLPCDDSSLL 940 ELRIVFEKGE 950 QENKNLPQAV 960 ASVKHQEHST 970 TACPAGLPCA 980 FFAPVPESLL 990 PLPVDDQQDA 1000 LGSEQPETLQ 1010 QTVVLQDPKS 1020 QIRAFRDPLQ 1030 DDTGLYVTEE 1040 VTSSEDKRKT 1050 YSLAFKQELK 1060 DVILCISPVI 1070 TFREPFLLTE 1080 KGMRCSTRDY 1090 FAEQVYWSPL 1100 LNKEFKEMEN 1110 RRKKQLLRDL 1120 SGLQGMNGSI 1130 QAKSIQVLPS 1140 HELVSTRIAE 1150 HLGDSQSLGR 1160 MLADYRARGG 1170 RIQPKNSDPF 1180 AHSKDASSTS 1190 SGQSGSKNEG 1200 DEERGLILSD 1210 AVWSTKVDCL 1220 NPINHQRLCV 1230 LFSSSSAQSS 1240 NAPSACVSPW 1250 IVTMEFYGKN 1260 DLTLGIFLER 1270 YCFRPSYQCP 1280 SMFCDTPMVH 1290 HIRRFVHGQG 1300 CVQIILKELD 1310 SPVPGYQHTI 1320 LTYSWCRICK 1330 QVTPVVALSN 1340 ESWSMSFAKY 1350 LELRFYGHQY 1360 TRRANAEPCG 1370 HSIHHDYHQY 1380 FSYNQMVASF 1390 SYSPIRLLEV 1400 CVPLPKIFIK 1410 RQAPLKVSLL 1420 QDLKDFFQKV 1430 SQVYVAIDER 1440 LASLKTDTFS 1450 KTREEKMEDI 1460 FAQKEMEEGE 1470 FKNWIEKMQA 1480 RLMSSSVDTP 1490 QQLQSVFESL 1500 IAKKQSLCEV 1510 LQAWNNRLQD 1520 LFQQEKGRKR 1530 PSVPPSPGRL 1540 RQGEESKISA 1550 MDASPRNISP 1560 GLQNGEKEDR 1570 FLTTLSSQSS 1580 TSSTHLQLPT 1590 PPEVMSEQSV 1600 GGPPELDTAS 1610 SSEDVFDGHL 1620 LGSTDSQVKE 1630 KSTMKAIFAN 1640 LLPGNSYNPI 1650 PFPFDPDKHY 1660 LMYEHERVPI 1670 AVCEKEPSSI 1680 IAFALSCKEY 1690 RNALEELSKA 1700 TQWNSAEEGL 1710 PTNSTSDSRP 1720 KSSSPIRLPE 1730 MSGGQTNRTT 1740 ETEPQPTKKA 1750 SGMLSFFRGT 1760 AGKSPDLSSQ 1770 KRETLRGADS 1780 AYYQVGQTGK 1790 EGTENQGVEP 1800 QDEVDGGDTQ 1810 KKQLINPHVE 1820 LQFSDANAKF 1830 YCRLYYAGEF 1840 HKMREVILDS 1850 SEEDFIRSLS 1860 HSSPWQARGG 1870 KSGAAFYATE 1880 DDRFILKQMP 1890 RLEVQSFLDF 1900 APHYFNYITN 1910 AVQQKRPTAL 1920 AKILGVYRIG 1930 YKNSQNNTEK 1940 KLDLLVMENL 1950 FYGRKMAQVF 1960 DLKGSLRNRN 1970 VKTDTGKESC 1980 DVVLLDENLL 1990 KMVRDNPLYI 2000 RSHSKAVLRT 2010 SIHSDSHFLS 2020 SHLIIDYSLL 2030 VGRDDTSNEL 2040 VVGIIDYIRT 2050 FTWDKKLEMV 2060 VKSTGILGGQ 2070 GKMPTVVSPE 2080 LYRTRFCEAM 2090 DKYFLMVPDH WTGLGLNC

Gene Ontology

Classification GO ID Description
Cellular Component GO:0031410 cytoplasmic vesicle
Cellular Component GO:0005829 cytosol
Cellular Component GO:0031901 early endosome membrane
Cellular Component GO:0010008 endosome membrane
Cellular Component GO:0000139 Golgi membrane
Cellular Component GO:0031902 late endosome membrane
Cellular Component GO:0045121 membrane raft
Cellular Component GO:0030670 phagocytic vesicle membrane
Cellular Component GO:0012506 vesicle membrane
Molecular Function GO:0000285 1-phosphatidylinositol-3-phosphate 5-kinase activity
Molecular Function GO:0016308 1-phosphatidylinositol-4-phosphate 5-kinase activity
Molecular Function GO:0052810 1-phosphatidylinositol-5-kinase activity
Molecular Function GO:0005524 ATP binding
Molecular Function GO:0043813 phosphatidylinositol-3,5-bisphosphate 5-phosphatase activity
Molecular Function GO:0106310 protein serine kinase activity
Molecular Function GO:0004674 protein serine/threonine kinase activity
Molecular Function GO:0008270 zinc ion binding
Biological Process GO:1903100 1-phosphatidyl-1D-myo-inositol 3,5-bisphosphate metabolic process
Biological Process GO:0019886 antigen processing and presentation of exogenous peptide antigen via MHC class II
Biological Process GO:0035556 intracellular signal transduction
Biological Process GO:0032438 melanosome organization
Biological Process GO:0030593 neutrophil chemotaxis
Biological Process GO:0036289 peptidyl-serine autophosphorylation
Biological Process GO:0090382 phagosome maturation
Biological Process GO:0090385 phagosome-lysosome fusion
Biological Process GO:1904562 phosphatidylinositol 5-phosphate metabolic process
Biological Process GO:0006661 phosphatidylinositol biosynthetic process
Biological Process GO:0034504 protein localization to nucleus
Biological Process GO:0006612 protein targeting to membrane
Biological Process GO:0019065 receptor-mediated endocytosis of virus by host cell
Biological Process GO:2000785 regulation of autophagosome assembly
Biological Process GO:1903426 regulation of reactive oxygen species biosynthetic process
Biological Process GO:0042147 retrograde transport, endosome to Golgi

Reference

[1] Wu Q, Li Z, Gong T, Zheng X, Zhou X et al.. Porphyromonas gingivalis infection induces lysine lactylation reprogramming in human umbilical vein endothelial cells.. Front Cell Infect Microbiol 16:1706727. 2026. PMID: 41696360.