Search Results

Overview

Uniprot IDQ9Y3S1
Protein NameSerine/threonine-protein kinase WNK2
Gene NameWNK2
OrganismHomo sapiens

Kla Sites from experimental identification

Position Flanking peptide
1780 QDVSSPAKTVGRFSV

Function

Serine/threonine-protein kinase component of the WNK2-SPAK/OSR1 kinase cascade, which plays an important role in the regulation of electrolyte homeostasis, cell signaling, survival, and proliferation (PubMed:17667937, PubMed:18593598, PubMed:21733846). The WNK2-SPAK/OSR1 kinase cascade is composed of WNK2, which mediates phosphorylation and activation of downstream kinases OXSR1/OSR1 and STK39/SPAK (By similarity). Following activation, OXSR1/OSR1 and STK39/SPAK catalyze phosphorylation of ion cotransporters, regulating their activity (By similarity). Acts as an activator and inhibitor of sodium-coupled chloride cotransporters and potassium-coupled chloride cotransporters respectively (PubMed:21733846). Activates SLC12A2, SCNN1A, SCNN1B, SCNN1D and SGK1 and inhibits SLC12A5 (PubMed:21733846). Negatively regulates the EGF-induced activation of the ERK/MAPK-pathway and the downstream cell cycle progression (PubMed:17667937, PubMed:18593598). Affects MAPK3/MAPK1 activity by modulating the activity of MAP2K1 and this modulation depends on phosphorylation of MAP2K1 by PAK1 (PubMed:17667937, PubMed:18593598). WNK2 acts by interfering with the activity of PAK1 by controlling the balance of the activity of upstream regulators of PAK1 activity, RHOA and RAC1, which display reciprocal activity (PubMed:17667937, PubMed:18593598)

Protein Sequence

10 MDGDGGRRDV 20 PGTLMEPGRG 30 AGPAGMAEPR 40 AKAARPGPQR 50 FLRRSVVESD 60 QEEPPGLEAA 70 EAPGPQPPQP 80 LQRRVLLLCK 90 TRRLIAERAR 100 GRPAAPAPAA 110 LVAQPGAPGA 120 PADAGPEPVG 130 TQEPGPDPIA 140 AAVETAPAPD 150 GGPREEAAAT 160 VRKEDEGAAE 170 AKPEPGRTRR 180 DEPEEEEDDE 190 DDLKAVATSL 200 DGRFLKFDIE 210 LGRGSFKTVY 220 KGLDTETWVE 230 VAWCELQDRK 240 LTKLERQRFK 250 EEAEMLKGLQ 260 HPNIVRFYDF 270 WESSAKGKRC 280 IVLVTELMTS 290 GTLKTYLKRF 300 KVMKPKVLRS 310 WCRQILKGLL 320 FLHTRTPPII 330 HRDLKCDNIF 340 ITGPTGSVKI 350 GDLGLATLKR 360 ASFAKSVIGT 370 PEFMAPEMYE 380 EHYDESVDVY 390 AFGMCMLEMA 400 TSEYPYSECQ 410 NAAQIYRKVT 420 CGIKPASFEK 430 VHDPEIKEII 440 GECICKNKEE 450 RYEIKDLLSH 460 AFFAEDTGVR 470 VELAEEDHGR 480 KSTIALRLWV 490 EDPKKLKGKP 500 KDNGAIEFTF 510 DLEKETPDEV 520 AQEMIESGFF 530 HESDVKIVAK 540 SIRDRVALIQ 550 WRRERIWPAL 560 QPKEQQDVGS 570 PDKARGPPVP 580 LQVQVTYHAQ 590 AGQPGPPEPE 600 EPEADQHLLP 610 PTLPTSATSL 620 ASDSTFDSGQ 630 GSTVYSDSQS 640 SQQSVMLGSL 650 ADAAPSPAQC 660 VCSPPVSEGP 670 VLPQSLPSLG 680 AYQQPTAAPG 690 LPVGSVPAPA 700 CPPSLQQHFP 710 DPAMSFAPVL 720 PPPSTPMPTG 730 PGQPAPPGQQ 740 PPPLAQPTPL 750 PQVLAPQPVV 760 PLQPVPPHLP 770 PYLAPASQVG 780 APAQLKPLQM 790 PQAPLQPLAQ 800 VPPQMPPIPV 810 VPPITPLAGI 820 DGLPPALPDL 830 PTATVPPVPP 840 PQYFSPAVIL 850 PSLAAPLPPA 860 SPALPLQAVK 870 LPHPPGAPLA 880 MPCRTIVPNA 890 PATIPLLAVA 900 PPGVAALSIH 910 SAVAQLPGQP 920 VYPAAFPQMA 930 PTDVPPSPHH 940 TVQNMRATPP 950 QPALPPQPTL 960 PPQPVLPPQP 970 TLPPQPVLPP 980 QPTRPPQPVL 990 PPQPMLPPQP 1000 VLPPQPALPV 1010 RPEPLQPHLP 1020 EQAAPAATPG 1030 SQILLGHPAP 1040 YAVDVAAQVP 1050 TVPVPPAAVL 1060 SPPLPEVLLP 1070 AAPELLPQFP 1080 SSLATVSASV 1090 QSVPTQTATL 1100 LPPANPPLPG 1110 GPGIASPCPT 1120 VQLTVEPVQE 1130 EQASQDKPPG 1140 LPQSCESYGG 1150 SDVTSGKELS 1160 DSCEGAFGGG 1170 RLEGRAARKH 1180 HRRSTRARSR 1190 QERASRPRLT 1200 ILNVCNTGDK 1210 MVECQLETHN 1220 HKMVTFKFDL 1230 DGDAPDEIAT 1240 YMVEHDFILQ 1250 AERETFIEQM 1260 KDVMDKAEDM 1270 LSEDTDADRG 1280 SDPGTSPPHL 1290 STCGLGTGEE 1300 SRQSQANAPV 1310 YQQNVLHTGK 1320 RWFIICPVAE 1330 HPAPEAPESS 1340 PPLPLSSLPP 1350 EASQGPCRGL 1360 TLPCLPWRRA 1370 ACGAVFLSLF 1380 SAESAQSKQP 1390 PDSAPYKDQL 1400 SSKEQPSFLA 1410 SQQLLSQAGP 1420 SNPPGAPPAP 1430 LAPSSPPVTA 1440 LPQDGAAPAT 1450 STMPEPASGT 1460 ASQAGGPGTP 1470 QGLTSELETS 1480 QPLAETHEAP 1490 LAVQPLVVGL 1500 APCTPAPEAA 1510 STRDASAPRE 1520 PLPPPAPEPS 1530 PHSGTPQPAL 1540 GQPAPLLPAA 1550 VGAVSLATSQ 1560 LPSPPLGPTV 1570 PPQPPSALES 1580 DGEGPPPRVG 1590 FVDSTIKSLD 1600 EKLRTLLYQE 1610 HVPTSSASAG 1620 TPVEVGDRDF 1630 TLEPLRGDQP 1640 RSEVCGGDLA 1650 LPPVPKEAVS 1660 GRVQLPQPLV 1670 EKSELAPTRG 1680 AVMEQGTSSS 1690 MTAESSPRSM 1700 LGYDRDGRQV 1710 ASDSHVVPSV 1720 PQDVPAFVRP 1730 ARVEPTDRDG 1740 GEAGESSAEP 1750 PPSDMGTVGG 1760 QASHPQTLGA 1770 RALGSPRKRP 1780 EQQDVSSPAK 1790 TVGRFSVVST 1800 QDEWTLASPH 1810 SLRYSAPPDV 1820 YLDEAPSSPD 1830 VKLAVRRAQT 1840 ASSIEVGVGE 1850 PVSSDSGDEG 1860 PRARPPVQKQ 1870 ASLPVSGSVA 1880 GDFVKKATAF 1890 LQRPSRAGSL 1900 GPETPSRVGM 1910 KVPTISVTSF 1920 HSQSSYISSD 1930 NDSELEDADI 1940 KKELQSLREK 1950 HLKEISELQS 1960 QQKQEIEALY 1970 RRLGKPLPPN 1980 VGFFHTAPPT 1990 GRRRKTSKSK 2000 LKAGKLLNPL 2010 VRQLKVVASS 2020 TGHLADSSRG 2030 PPAKDPAQAS 2040 VGLTADSTGL 2050 SGKAVQTQQP 2060 CSVRASLSSD 2070 ICSGLASDGG 2080 GARGQGWTVY 2090 HPTSERVTYK 2100 SSSKPRARFL 2110 SGPVSVSIWS 2120 ALKRLCLGKE 2130 HSSRSSTSSL 2140 APGPEPGPQP 2150 ALHVQAQVNN 2160 SNNKKGTFTD 2170 DLHKLVDEWT 2180 SKTVGAAQLK 2190 PTLNQLKQTQ 2200 KLQDMEAQAG 2210 WAAPGEARAM 2220 TAPRAGVGMP 2230 RLPPAPGPLS 2240 TTVIPGAAPT 2250 LSVPTPDGAL 2260 GTARRNQVWF 2270 GLRVPPTACC 2280 GHSTQPRGGQ 2290 RVGSKTASFA ASDPVRS

Gene Ontology

Classification GO ID Description
Cellular Component GO:0005737 cytoplasm
Cellular Component GO:0005829 cytosol
Cellular Component GO:0005886 plasma membrane
Molecular Function GO:0005524 ATP binding
Molecular Function GO:0106310 protein serine kinase activity
Molecular Function GO:0004674 protein serine/threonine kinase activity
Biological Process GO:0006974 DNA damage response
Biological Process GO:0035556 intracellular signal transduction
Biological Process GO:0050801 monoatomic ion homeostasis
Biological Process GO:0008285 negative regulation of cell population proliferation
Biological Process GO:0070373 negative regulation of ERK1 and ERK2 cascade
Biological Process GO:0090263 positive regulation of canonical Wnt signaling pathway
Biological Process GO:2000651 positive regulation of sodium ion transmembrane transporter activity
Biological Process GO:0046777 protein autophosphorylation
Biological Process GO:0006468 protein phosphorylation
Biological Process GO:1904062 regulation of monoatomic cation transmembrane transport

Reference

PMID: N/A