Search Results

Overview

Uniprot ID	Q9Y4B6
Protein Name	DDB1- and CUL4-associated factor 1
Gene Name	DCAF1
Organism	Homo sapiens

Kla Sites from experimental identification

Position	Flanking peptide
265	KPEDGGLKKNKSAKQ
284	NFRKAKQKLGFSSSD
701	NRISSIGKFISGTPR
710	ISGTPRRKLPQNPKS
716	RKLPQNPKSSEHTLA

Function

Acts both as a substrate recognition component of E3 ubiquitin-protein ligase complexes and as an atypical serine/threonine-protein kinase, playing key roles in various processes such as cell cycle, telomerase regulation and histone modification. Probable substrate-specific adapter of a DCX (DDB1-CUL4-X-box) E3 ubiquitin-protein ligase complex, named CUL4A-RBX1-DDB1-DCAF1/VPRBP complex, which mediates ubiquitination and proteasome-dependent degradation of proteins such as NF2 (PubMed:23063525). Involved in the turnover of methylated proteins: recognizes and binds methylated proteins via its chromo domain, leading to ubiquitination of target proteins by the RBX1-DDB1-DCAF1/VPRBP complex (PubMed:23063525). The CUL4A-RBX1-DDB1-DCAF1/VPRBP complex is also involved in B-cell development: DCAF1 is recruited by RAG1 to ubiquitinate proteins, leading to limit error-prone repair during V(D)J recombination (By similarity). Also part of the EDVP complex, an E3 ligase complex that mediates ubiquitination of proteins such as TERT, leading to TERT degradation and telomerase inhibition (PubMed:19287380, PubMed:23362280). The EDVP complex also mediates ubiquitination and degradation of CCP110 (PubMed:28242748, PubMed:34259627). Also acts as an atypical serine/threonine-protein kinase that specifically mediates phosphorylation of 'Thr-120' of histone H2A (H2AT120ph) in a nucleosomal context, thereby repressing transcription (PubMed:24140421). H2AT120ph is present in the regulatory region of many tumor suppresor genes, down-regulates their transcription and is present at high level in a number of tumors (PubMed:24140421). Involved in JNK-mediated apoptosis during cell competition process via its interaction with LLGL1 and LLGL2 (PubMed:20644714). By acting on TET dioxygenses, essential for oocyte maintenance at the primordial follicle stage, hence essential for female fertility (By similarity)

Protein Sequence

10 MTTVVVHVDS 20 KAELTTLLEQ 30 WEKEHGSGQD 40 MVPILTRMSQ 50 LIEKETEEYR 60 KGDPDPFDDR 70 HPGRADPECM 80 LGHLLRILFK 90 NDDFMNALVN 100 AYVMTSREPP 110 LNTAACRLLL 120 DIMPGLETAV 130 VFQEKEGIVE 140 NLFKWAREAD 150 QPLRTYSTGL 160 LGGAMENQDI 170 AANYRDENSQ 180 LVAIVLRRLR 190 ELQLQEVALR 200 QENKRPSPRK 210 LSSEPLLPLD 220 EEAVDMDYGD 230 MAVDVVDGDQ 240 EEASGDMEIS 250 FHLDSGHKTS 260 SRVNSTTKPE 270 DGGLKKNKSA 280 KQGDRENFRK 290 AKQKLGFSSS 300 DPDRMFVELS 310 NSSWSEMSPW 320 VIGTNYTLYP 330 MTPAIEQRLI 340 LQYLTPLGEY 350 QELLPIFMQL 360 GSRELMMFYI 370 DLKQTNDVLL 380 TFEALKHLAS 390 LLLHNKFATE 400 FVAHGGVQKL 410 LEIPRPSMAA 420 TGVSMCLYYL 430 SYNQDAMERV 440 CMHPHNVLSD 450 VVNYTLWLME 460 CSHASGCCHA 470 TMFFSICFSF 480 RAVLELFDRY 490 DGLRRLVNLI 500 STLEILNLED 510 QGALLSDDEI 520 FASRQTGKHT 530 CMALRKYFEA 540 HLAIKLEQVK 550 QSLQRTEGGI 560 LVHPQPPYKA 570 CSYTHEQIVE 580 MMEFLIEYGP 590 AQLYWEPAEV 600 FLKLSCVQLL 610 LQLISIACNW 620 KTYYARNDTV 630 RFALDVLAIL 640 TVVPKIQLQL 650 AESVDVLDEA 660 GSTVSTVGIS 670 IILGVAEGEF 680 FIHDAEIQKS 690 ALQIIINCVC 700 GPDNRISSIG 710 KFISGTPRRK 720 LPQNPKSSEH 730 TLAKMWNVVQ 740 SNNGIKVLLS 750 LLSIKMPITD 760 ADQIRALACK 770 ALVGLSRSST 780 VRQIISKLPL 790 FSSCQIQQLM 800 KEPVLQDKRS 810 DHVKFCKYAA 820 ELIERVSGKP 830 LLIGTDVSLA 840 RLQKADVVAQ 850 SRISFPEKEL 860 LLLIRNHLIS 870 KGLGETATVL 880 TKEADLPMTA 890 ASHSSAFTPV 900 TAAASPVSLP 910 RTPRIANGIA 920 TRLGSHAAVG 930 ASAPSAPTAH 940 PQPRPPQGPL 950 ALPGPSYAGN 960 SPLIGRISFI 970 RERPSPCNGR 980 KIRVLRQKSD 990 HGAYSQSPAI 1000 KKQLDRHLPS 1010 PPTLDSIITE 1020 YLREQHARCK 1030 NPVATCPPFS 1040 LFTPHQCPEP 1050 KQRRQAPINF 1060 TSRLNRRASF 1070 PKYGGVDGGC 1080 FDRHLIFSRF 1090 RPISVFREAN 1100 EDESGFTCCA 1110 FSARERFLML 1120 GTCTGQLKLY 1130 NVFSGQEEAS 1140 YNCHNSAITH 1150 LEPSRDGSLL 1160 LTSATWSQPL 1170 SALWGMKSVF 1180 DMKHSFTEDH 1190 YVEFSKHSQD 1200 RVIGTKGDIA 1210 HIYDIQTGNK 1220 LLTLFNPDLA 1230 NNYKRNCATF 1240 NPTDDLVLND 1250 GVLWDVRSAQ 1260 AIHKFDKFNM 1270 NISGVFHPNG 1280 LEVIINTEIW 1290 DLRTFHLLHT 1300 VPALDQCRVV 1310 FNHTGTVMYG 1320 AMLQADDEDD 1330 LMEERMKSPF 1340 GSSFRTFNAT 1350 DYKPIATIDV 1360 KRNIFDLCTD 1370 TKDCYLAVIE 1380 NQGSMDALNM 1390 DTVCRLYEVG 1400 RQRLAEDEDE 1410 EEDQEEEEQE 1420 EEDDDEDDDD 1430 TDDLDELDTD 1440 QLLEAELEED 1450 DNNENAGEDG 1460 DNDFSPSDEE 1470 LANLLEEGED 1480 GEDEDSDADE 1490 EVELILGDTD 1500 SSDNSDLEDD IILSLNE

Gene Ontology

Classification	GO ID	Description
Cellular Component	GO:0005813	centrosome
Cellular Component	GO:0080008	Cul4-RING E3 ubiquitin ligase complex
Cellular Component	GO:0005737	cytoplasm
Cellular Component	GO:0001650	fibrillar center
Cellular Component	GO:0005654	nucleoplasm
Cellular Component	GO:0005634	nucleus
Molecular Function	GO:0005524	ATP binding
Molecular Function	GO:1990244	histone H2AT120 kinase activity
Molecular Function	GO:0030331	nuclear estrogen receptor binding
Molecular Function	GO:0106310	protein serine kinase activity
Molecular Function	GO:1990756	ubiquitin-like ligase-substrate adaptor activity
Biological Process	GO:0030183	B cell differentiation
Biological Process	GO:0035212	cell competition in a multicellular organism
Biological Process	GO:0006351	DNA-templated transcription
Biological Process	GO:0000122	negative regulation of transcription by RNA polymerase II
Biological Process	GO:0045732	positive regulation of protein catabolic process
Biological Process	GO:0043687	post-translational protein modification
Biological Process	GO:0043161	proteasome-mediated ubiquitin-dependent protein catabolic process
Biological Process	GO:0016567	protein ubiquitination
Biological Process	GO:0033151	V(D)J recombination

Reference

[1] Yang D, Yin J, Shan L, Yi X, Zhang W et al.. Identification of lysine-lactylated substrates in gastric cancer cells.. iScience 25(7):104630. 2022 Jul 15. PMID: 35800753.

[2] He C, Zhang J, Bai X, Lu C, Zhang K. Lysine lactylation-based insight to understanding the characterization of cervical cancer.. Biochim Biophys Acta Mol Basis Dis 1870(7):167356. 2024 Oct. PMID: 39025375.

[3] He J, Lai T, Zhou Z, Yang H, Lei Z et al.. Multiomics profiling reveals the involvement of protein lactylation in nonhomologous end joining pathway conferring radioresistance in lung adenocarcinoma cell.. Sci Rep 15(1):24651. 2025 Jul 9. PMID: 40634431.

[4] Wu Q, Li Z, Gong T, Zheng X, Zhou X et al.. Porphyromonas gingivalis infection induces lysine lactylation reprogramming in human umbilical vein endothelial cells.. Front Cell Infect Microbiol 16:1706727. 2026. PMID: 41696360.