Search Results

Overview

Uniprot IDQ9Y606
Protein NamePseudouridylate synthase 1 homolog
Gene NamePUS1
OrganismHomo sapiens

Kla Sites from experimental identification

Position Flanking peptide
147 QRCARTDKGVSAAGQ
67 EDGEHPAKKLKSGGD
68 DGEHPAKKLKSGGDE

Function

Pseudouridylate synthase that catalyzes pseudouridylation of tRNAs and mRNAs (PubMed:15772074, PubMed:24722331). Acts on positions 27/28 in the anticodon stem and also positions 34 and 36 in the anticodon of an intron containing tRNA (PubMed:24722331). Also catalyzes pseudouridylation of mRNAs: mediates pseudouridylation of mRNAs with the consensus sequence 5'-UGUAG-3' (PubMed:31477916, PubMed:35051350). Acts as a regulator of pre-mRNA splicing by mediating pseudouridylation of pre-mRNAs at locations associated with alternatively spliced regions (PubMed:35051350). Pseudouridylation of pre-mRNAs near splice sites directly regulates mRNA splicing and mRNA 3'-end processing (PubMed:35051350). Involved in regulation of nuclear receptor activity through pseudouridylation of SRA1 mRNA (PubMed:24722331)

Protein Sequence

10 MGLQLRALLG 20 AFGRWTLRLG 30 PRPSCSPRMA 40 GNAEPPPAGA 50 ACPQDRRSCS 60 GRAGGDRVWE 70 DGEHPAKKLK 80 SGGDEERREK 90 PPKRKIVLLM 100 AYSGKGYHGM 110 QRNVGSSQFK 120 TIEDDLVSAL 130 VRSGCIPENH 140 GEDMRKMSFQ 150 RCARTDKGVS 160 AAGQVVSLKV 170 WLIDDILEKI 180 NSHLPSHIRI 190 LGLKRVTGGF 200 NSKNRCDART 210 YCYLLPTFAF 220 AHKDRDVQDE 230 TYRLSAETLQ 240 QVNRLLACYK 250 GTHNFHNFTS 260 QKGPQDPSAC 270 RYILEMYCEE 280 PFVREGLEFA 290 VIRVKGQSFM 300 MHQIRKMVGL 310 VVAIVKGYAP 320 ESVLERSWGT 330 EKVDVPKAPG 340 LGLVLERVHF 350 EKYNQRFGND 360 GLHEPLDWAQ 370 EEGKVAAFKE 380 EHIYPTIIGT 390 ERDERSMAQW 400 LSTLPIHNFS 410 ATALTAGGTG 420 AKVPSPLEGS EGDGDTD

Gene Ontology

Classification GO ID Description
Cellular Component GO:0005737 cytoplasm
Biological Process GO:0031119 tRNA pseudouridine synthesis
Cellular Component GO:0005759 mitochondrial matrix
Cellular Component GO:0005739 mitochondrion
Cellular Component GO:0005730 nucleolus
Cellular Component GO:0005654 nucleoplasm
Cellular Component GO:0005634 nucleus
Cellular Component GO:0005667 transcription regulator complex
Molecular Function GO:0003682 chromatin binding
Molecular Function GO:0160153 mitochondrial tRNA pseudouridine(27/28) synthase activity
Molecular Function GO:0009982 pseudouridine synthase activity
Molecular Function GO:0003723 RNA binding
Molecular Function GO:0002153 steroid receptor RNA activator RNA binding
Molecular Function GO:0003713 transcription coactivator activity
Molecular Function GO:0000049 tRNA binding
Molecular Function GO:0160147 tRNA pseudouridine(38-40) synthase activity
Biological Process GO:0070902 mitochondrial tRNA pseudouridine synthesis
Biological Process GO:0006397 mRNA processing
Biological Process GO:1990481 mRNA pseudouridine synthesis
Biological Process GO:0008380 RNA splicing

Reference

[1] Yang Z, Yan C, Ma J, Peng P, Ren X et al.. Lactylome analysis suggests lactylation-dependent mechanisms of metabolic adaptation in hepatocellular carcinoma.. Nat Metab 5(1):61-79. 2023 Jan. PMID: 36593272.

[2] Guo X, Ren X, Yan C, Huang H. Quantitative Proteomics Reveals the Role of Lysine Lactylation in Lenalidomide-Resistance in Multiple Myeloma Cells.. ACS Chem Biol 20(7):1728-1738. 2025 Jul 18. PMID: 40590393.

[3] Wu Q, Li Z, Gong T, Zheng X, Zhou X et al.. Porphyromonas gingivalis infection induces lysine lactylation reprogramming in human umbilical vein endothelial cells.. Front Cell Infect Microbiol 16:1706727. 2026. PMID: 41696360.