Search Results

Overview

Uniprot IDQ9Y617
Protein NamePhosphoserine aminotransferase
Gene NamePSAT1
OrganismHomo sapiens

Kla Sites from experimental identification

Position Flanking peptide
117 KAAEEAKKFGTINIV
300 VEPQNRSKMNIPFRI
311 PFRIGNAKGDDALEK
33 QKELLDYKGVGISVL
333 ELNMLSLKGHRSVGG
363 KLAAFMKKFLEMHQL

Function

Involved in L-serine biosynthesis via the phosphorylated pathway, a three-step pathway converting the glycolytic intermediate 3-phospho-D-glycerate into L-serine (PubMed:36851825, PubMed:37627284). Catalyzes the second step, that is the pyridoxal 5'-phosphate-dependent transamination of 3-phosphohydroxypyruvate and L-glutamate to O-phosphoserine (OPS) and alpha-ketoglutarate (PubMed:36851825, PubMed:37627284). Acts as an inhibitor of ferroptosis in response to interferon-gamma (IFNG) by promoting GPX4 stability: following phosphorylation by CAMK2A, PSAT1 interacts with GPX4 and provides 2-oxoglutarate to EGLN3, leading to GPX4 hydroxylation and stability (PubMed:40281343)

Protein Sequence

10 MDAPRQVVNF 20 GPGPAKLPHS 30 VLLEIQKELL 40 DYKGVGISVL 50 EMSHRSSDFA 60 KIINNTENLV 70 RELLAVPDNY 80 KVIFLQGGGC 90 GQFSAVPLNL 100 IGLKAGRCAD 110 YVVTGAWSAK 120 AAEEAKKFGT 130 INIVHPKLGS 140 YTKIPDPSTW 150 NLNPDASYVY 160 YCANETVHGV 170 EFDFIPDVKG 180 AVLVCDMSSN 190 FLSKPVDVSK 200 FGVIFAGAQK 210 NVGSAGVTVV 220 IVRDDLLGFA 230 LRECPSVLEY 240 KVQAGNSSLY 250 NTPPCFSIYV 260 MGLVLEWIKN 270 NGGAAAMEKL 280 SSIKSQTIYE 290 IIDNSQGFYV 300 CPVEPQNRSK 310 MNIPFRIGNA 320 KGDDALEKRF 330 LDKALELNML 340 SLKGHRSVGG 350 IRASLYNAVT 360 IEDVQKLAAF 370 MKKFLEMHQL

Gene Ontology

Classification GO ID Description
Cellular Component GO:0005737 cytoplasm
Cellular Component GO:0005829 cytosol
Cellular Component GO:0070062 extracellular exosome
Molecular Function GO:0004648 O-phospho-L-serine:2-oxoglutarate aminotransferase activity
Molecular Function GO:0030170 pyridoxal phosphate binding
Biological Process GO:0006564 L-serine biosynthetic process
Biological Process GO:0110076 negative regulation of ferroptosis
Biological Process GO:0008615 pyridoxine biosynthetic process

Reference

[1] Yang Z, Yan C, Ma J, Peng P, Ren X et al.. Lactylome analysis suggests lactylation-dependent mechanisms of metabolic adaptation in hepatocellular carcinoma.. Nat Metab 5(1):61-79. 2023 Jan. PMID: 36593272.

[2] Shi CM, Wang QC, Li XL, Yang YH, Tang XY et al.. Global Profiling of Protein Lactylation in Human Hippocampi.. Proteomics Clin Appl 19(2):e202400061. 2025 Mar. PMID: 39610256.