Search Results

Overview

Uniprot ID	Q9Y6D9
Protein Name	Mitotic spindle assembly checkpoint protein MAD1
Gene Name	MAD1L1
Organism	Homo sapiens

Kla Sites from experimental identification

Position	Flanking peptide
61	RAEQIRSKSHLIQVE

Function

Component of the spindle-assembly checkpoint that prevents the onset of anaphase until all chromosomes are properly aligned at the metaphase plate (PubMed:10049595, PubMed:20133940, PubMed:29162720). Forms a heterotetrameric complex with the closed conformation form of MAD2L1 (C-MAD2) at unattached kinetochores during prometaphase, recruits an open conformation of MAD2L1 (O-MAD2) and promotes the conversion of O-MAD2 to C-MAD2, which ensures mitotic checkpoint signaling (PubMed:29162720)

Protein Sequence

10 MEDLGENTMV 20 LSTLRSLNNF 30 ISQRVEGGSG 40 LDISTSAPGS 50 LQMQYQQSMQ 60 LEERAEQIRS 70 KSHLIQVERE 80 KMQMELSHKR 90 ARVELERAAS 100 TSARNYEREV 110 DRNQELLTRI 120 RQLQEREAGA 130 EEKMQEQLER 140 NRQCQQNLDA 150 ASKRLREKED 160 SLAQAGETIN 170 ALKGRISELQ 180 WSVMDQEMRV 190 KRLESEKQEL 200 QEQLDLQHKK 210 CQEANQKIQE 220 LQASQEARAD 230 HEQQIKDLEQ 240 KLSLQEQDAA 250 IVKNMKSELV 260 RLPRLERELK 270 QLREESAHLR 280 EMRETNGLLQ 290 EELEGLQRKL 300 GRQEKMQETL 310 VGLELENERL 320 LAKLQSWERL 330 DQTMGLSIRT 340 PEDLSRFVVE 350 LQQRELALKD 360 KNSAVTSSAR 370 GLEKARQQLQ 380 EELRQVSGQL 390 LEERKKRETH 400 EALARRLQKR 410 VLLLTKERDG 420 MRAILGSYDS 430 ELTPAEYSPQ 440 LTRRMREAED 450 MVQKVHSHSA 460 EMEAQLSQAL 470 EELGGQKQRA 480 DMLEMELKML 490 KSQSSSAEQS 500 FLFSREEADT 510 LRLKVEELEG 520 ERSRLEEEKR 530 MLEAQLERRA 540 LQGDYDQSRT 550 KVLHMSLNPT 560 SVARQRLRED 570 HSQLQAECER 580 LRGLLRAMER 590 GGTVPADLEA 600 AAASLPSSKE 610 VAELKKQVES 620 AELKNQRLKE 630 VFQTKIQEFR 640 KACYTLTGYQ 650 IDITTENQYR 660 LTSLYAEHPG 670 DCLIFKATSP 680 SGSKMQLLET 690 EFSHTVGELI 700 EVHLRRQDSI 710 PAFLSSLTLE LFSRQTVA

Gene Ontology

Classification	GO ID	Description
Cellular Component	GO:0005813	centrosome
Cellular Component	GO:0005737	cytoplasm
Cellular Component	GO:0005829	cytosol
Cellular Component	GO:0000776	kinetochore
Cellular Component	GO:1990706	MAD1 complex
Cellular Component	GO:0072686	mitotic spindle
Cellular Component	GO:1990728	mitotic spindle assembly checkpoint MAD1-MAD2 complex
Cellular Component	GO:0005635	nuclear envelope
Cellular Component	GO:0031965	nuclear membrane
Cellular Component	GO:0044615	nuclear pore nuclear basket
Cellular Component	GO:0005654	nucleoplasm
Cellular Component	GO:0005634	nucleus
Cellular Component	GO:0005819	spindle
Cellular Component	GO:0000922	spindle pole
Molecular Function	GO:0043515	kinetochore binding
Biological Process	GO:0051315	attachment of mitotic spindle microtubules to kinetochore
Biological Process	GO:0051301	cell division
Biological Process	GO:1902426	deactivation of mitotic spindle assembly checkpoint
Biological Process	GO:0007094	mitotic spindle assembly checkpoint signaling
Biological Process	GO:0090267	positive regulation of mitotic cell cycle spindle assembly checkpoint
Biological Process	GO:0090235	regulation of metaphase plate congression

Reference

[1] He J, Lai T, Zhou Z, Yang H, Lei Z et al.. Multiomics profiling reveals the involvement of protein lactylation in nonhomologous end joining pathway conferring radioresistance in lung adenocarcinoma cell.. Sci Rep 15(1):24651. 2025 Jul 9. PMID: 40634431.

[2] Wu Q, Li Z, Gong T, Zheng X, Zhou X et al.. Porphyromonas gingivalis infection induces lysine lactylation reprogramming in human umbilical vein endothelial cells.. Front Cell Infect Microbiol 16:1706727. 2026. PMID: 41696360.