Overview
| Uniprot ID | Q9Y6K1 |
|---|
| Protein Name | DNA (cytosine-5)-methyltransferase 3A |
|---|
| Gene Name | DNMT3A |
|---|
| Organism | Homo sapiens |
|---|
Kla Sites from experimental identification
| Position |
Flanking peptide |
| 200 |
GDPYYISKRKRDEWL |
| 577 |
GAAQAAIKEDPWNCY |
Function
Required for genome-wide de novo methylation and is essential for the establishment of DNA methylation patterns during development (PubMed:12138111, PubMed:16357870, PubMed:30478443). DNA methylation is coordinated with methylation of histones (PubMed:12138111, PubMed:16357870, PubMed:30478443). It modifies DNA in a non-processive manner and also methylates non-CpG sites (PubMed:12138111, PubMed:16357870, PubMed:30478443). May preferentially methylate DNA linker between 2 nucleosomal cores and is inhibited by histone H1 (By similarity). Plays a role in paternal and maternal imprinting (By similarity). Required for methylation of most imprinted loci in germ cells (By similarity). Acts as a transcriptional corepressor for ZBTB18 (By similarity). Recruited to trimethylated 'Lys-36' of histone H3 (H3K36me3) sites (By similarity). Can actively repress transcription through the recruitment of HDAC activity (By similarity). Also has weak auto-methylation activity on Cys-710 in absence of DNA (By similarity)
Protein Sequence
10
MPAMPSSGPG
20
DTSSSAAERE
30
EDRKDGEEQE
40
EPRGKEERQE
50
PSTTARKVGR
60
PGRKRKHPPV
70
ESGDTPKDPA
80
VISKSPSMAQ
90
DSGASELLPN
100
GDLEKRSEPQ
110
PEEGSPAGGQ
120
KGGAPAEGEG
130
AAETLPEASR
140
AVENGCCTPK
150
EGRGAPAEAG
160
KEQKETNIES
170
MKMEGSRGRL
180
RGGLGWESSL
190
RQRPMPRLTF
200
QAGDPYYISK
210
RKRDEWLARW
220
KREAEKKAKV
230
IAGMNAVEEN
240
QGPGESQKVE
250
EASPPAVQQP
260
TDPASPTVAT
270
TPEPVGSDAG
280
DKNATKAGDD
290
EPEYEDGRGF
300
GIGELVWGKL
310
RGFSWWPGRI
320
VSWWMTGRSR
330
AAEGTRWVMW
340
FGDGKFSVVC
350
VEKLMPLSSF
360
CSAFHQATYN
370
KQPMYRKAIY
380
EVLQVASSRA
390
GKLFPVCHDS
400
DESDTAKAVE
410
VQNKPMIEWA
420
LGGFQPSGPK
430
GLEPPEEEKN
440
PYKEVYTDMW
450
VEPEAAAYAP
460
PPPAKKPRKS
470
TAEKPKVKEI
480
IDERTRERLV
490
YEVRQKCRNI
500
EDICISCGSL
510
NVTLEHPLFV
520
GGMCQNCKNC
530
FLECAYQYDD
540
DGYQSYCTIC
550
CGGREVLMCG
560
NNNCCRCFCV
570
ECVDLLVGPG
580
AAQAAIKEDP
590
WNCYMCGHKG
600
TYGLLRRRED
610
WPSRLQMFFA
620
NNHDQEFDPP
630
KVYPPVPAEK
640
RKPIRVLSLF
650
DGIATGLLVL
660
KDLGIQVDRY
670
IASEVCEDSI
680
TVGMVRHQGK
690
IMYVGDVRSV
700
TQKHIQEWGP
710
FDLVIGGSPC
720
NDLSIVNPAR
730
KGLYEGTGRL
740
FFEFYRLLHD
750
ARPKEGDDRP
760
FFWLFENVVA
770
MGVSDKRDIS
780
RFLESNPVMI
790
DAKEVSAAHR
800
ARYFWGNLPG
810
MNRPLASTVN
820
DKLELQECLE
830
HGRIAKFSKV
840
RTITTRSNSI
850
KQGKDQHFPV
860
FMNEKEDILW
870
CTEMERVFGF
880
PVHYTDVSNM
890
SRLARQRLLG
900
RSWSVPVIRH
910
LFAPLKEYFA
CV
Gene Ontology
| Classification |
GO ID |
Description |
| Cellular Component |
GO:0000775 |
chromosome, centromeric region |
| Cellular Component |
GO:0005737 |
cytoplasm |
| Cellular Component |
GO:0000791 |
euchromatin |
| Cellular Component |
GO:0000792 |
heterochromatin |
| Cellular Component |
GO:0016363 |
nuclear matrix |
| Cellular Component |
GO:0005654 |
nucleoplasm |
| Cellular Component |
GO:0005634 |
nucleus |
| Cellular Component |
GO:0001741 |
XY body |
| Molecular Function |
GO:0003682 |
chromatin binding |
| Molecular Function |
GO:0003886 |
DNA (cytosine-5-)-methyltransferase activity |
| Molecular Function |
GO:0003677 |
DNA binding |
| Molecular Function |
GO:0106222 |
lncRNA binding |
| Molecular Function |
GO:0106363 |
protein-cysteine methyltransferase activity |
| Molecular Function |
GO:0000978 |
RNA polymerase II cis-regulatory region sequence-specific DNA binding |
| Molecular Function |
GO:0061629 |
RNA polymerase II-specific DNA-binding transcription factor binding |
| Molecular Function |
GO:0003714 |
transcription corepressor activity |
| Molecular Function |
GO:0045322 |
unmethylated CpG binding |
| Molecular Function |
GO:0008270 |
zinc ion binding |
| Biological Process |
GO:0071361 |
cellular response to ethanol |
| Biological Process |
GO:0071456 |
cellular response to hypoxia |
| Biological Process |
GO:0006346 |
DNA methylation-dependent constitutive heterochromatin formation |
| Biological Process |
GO:0097284 |
hepatocyte apoptotic process |
| Biological Process |
GO:0032259 |
methylation |
| Biological Process |
GO:0045892 |
negative regulation of DNA-templated transcription |
| Biological Process |
GO:0044027 |
negative regulation of gene expression via chromosomal CpG island methylation |
| Biological Process |
GO:0000122 |
negative regulation of transcription by RNA polymerase II |
| Biological Process |
GO:0030182 |
neuron differentiation |
| Biological Process |
GO:1900039 |
positive regulation of cellular response to hypoxia |
| Biological Process |
GO:0031048 |
regulatory ncRNA-mediated heterochromatin formation |
| Biological Process |
GO:0042220 |
response to cocaine |
| Biological Process |
GO:0032355 |
response to estradiol |
| Biological Process |
GO:0010212 |
response to ionizing radiation |
| Biological Process |
GO:0010288 |
response to lead ion |
| Biological Process |
GO:0009636 |
response to toxic substance |
| Biological Process |
GO:0033189 |
response to vitamin A |
| Biological Process |
GO:0009410 |
response to xenobiotic stimulus |
| Biological Process |
GO:0141196 |
transposable element silencing by piRNA-mediated DNA methylation |
Reference
[1] He J, Lai T, Zhou Z, Yang H, Lei Z et al.. Multiomics profiling reveals the involvement of protein lactylation in nonhomologous end joining pathway conferring radioresistance in lung adenocarcinoma cell.. Sci Rep 15(1):24651. 2025 Jul 9. PMID: 40634431.