Search Results

Overview

Uniprot ID	Q9Z0X1
Protein Name	Apoptosis-inducing factor 1, mitochondrial
Gene Name	Aifm1
Organism	Mus musculus

Kla Sites from experimental identification

Position	Flanking peptide
592	PIARKIIKDGEQHED

Function

Functions both as NADH oxidoreductase and as regulator of apoptosis (By similarity). In response to apoptotic stimuli, it is released from the mitochondrion intermembrane space into the cytosol and to the nucleus, where it functions as a proapoptotic factor in a caspase-independent pathway (PubMed:12114629, PubMed:21467298, PubMed:9989411). Release into the cytoplasm is mediated upon binding to poly-ADP-ribose chains (PubMed:21467298). The soluble form (AIFsol) found in the nucleus induces 'parthanatos' i.e. caspase-independent fragmentation of chromosomal DNA (PubMed:12114629, PubMed:21467298, PubMed:9989411). Binds to DNA in a sequence-independent manner (PubMed:21467298). Interacts with EIF3G, and thereby inhibits the EIF3 machinery and protein synthesis, and activates caspase-7 to amplify apoptosis (By similarity). Plays a critical role in caspase-independent, pyknotic cell death in hydrogen peroxide-exposed cells (By similarity). In contrast, participates in normal mitochondrial metabolism. Plays an important role in the regulation of respiratory chain biogenesis by interacting with CHCHD4 and controlling CHCHD4 mitochondrial import (PubMed:19447115)

Protein Sequence

10 MFRCGGLAGA 20 FKQKLVPLVR 30 TVYVQRPKQR 40 NRLPGNLFQQ 50 WRVPLELQMA 60 RQMASSGSSG 70 GKMDNSVLVL 80 IVGLSTIGAG 90 AYAYKTIKED 100 QKRYNERVMG 110 LGLSPEEKQR 120 RAIASATEGG 130 SVPQIRAPSH 140 VPFLLIGGGT 150 AAFAAARSIR 160 ARDPGARVLI 170 VSEDPELPYM 180 RPPLSKELWF 190 SDDPNVTKTL 200 QFRQWNGKER 210 SIYFQPPSFY 220 VSAQDLPNIE 230 NGGVAVLTGK 240 KVVHLDVRGN 250 MVKLNDGSQI 260 TFEKCLIATG 270 GTPRSLSAID 280 RAGAEVKSRT 290 TLFRKIGDFR 300 ALEKISREVK 310 SITVIGGGFL 320 GSELACALGR 330 KSQASGIEVI 340 QLFPEKGNMG 350 KILPQYLSNW 360 TMEKVKREGV 370 KVMPNAIVQS 380 VGVSGGRLLI 390 KLKDGRKVET 400 DHIVTAVGLE 410 PNVELAKTGG 420 LEIDSDFGGF 430 RVNAELQARS 440 NIWVAGDAAC 450 FYDIKLGRRR 460 VEHHDHAVVS 470 GRLAGENMTG 480 AAKPYWHQSM 490 FWSDLGPDVG 500 YEAIGLVDSS 510 LPTVGVFAKA 520 TAQDNPKSAT 530 EQSGTGIRSE 540 SETESEASEI 550 TIPPSAPAVP 560 QVPVEGEDYG 570 KGVIFYLRDK 580 VVVGIVLWNV 590 FNRMPIARKI 600 IKDGEQHEDL 610 NEVAKLFNIH ED

Gene Ontology

Classification	GO ID	Description
Cellular Component	GO:0005737	cytoplasm
Cellular Component	GO:0005829	cytosol
Cellular Component	GO:0005743	mitochondrial inner membrane
Cellular Component	GO:0005758	mitochondrial intermembrane space
Cellular Component	GO:0005741	mitochondrial outer membrane
Cellular Component	GO:0005739	mitochondrion
Cellular Component	GO:0005634	nucleus
Cellular Component	GO:0048471	perinuclear region of cytoplasm
Cellular Component	GO:0120212	sperm head-tail coupling apparatus
Molecular Function	GO:0003677	DNA binding
Molecular Function	GO:0004174	electron-transferring-flavoprotein dehydrogenase activity
Molecular Function	GO:0071949	FAD binding
Molecular Function	GO:0016174	NAD(P)H oxidase H2O2-forming activity
Molecular Function	GO:0003954	NADH dehydrogenase activity
Molecular Function	GO:0016651	oxidoreductase activity, acting on NAD(P)H
Molecular Function	GO:0072572	poly-ADP-D-ribose binding
Molecular Function	GO:0046983	protein dimerization activity
Biological Process	GO:0008637	apoptotic mitochondrial changes
Biological Process	GO:0006915	apoptotic process
Biological Process	GO:0070059	intrinsic apoptotic signaling pathway in response to endoplasmic reticulum stress
Biological Process	GO:0160203	mitochondrial disulfide relay system
Biological Process	GO:0033108	mitochondrial respiratory chain complex assembly
Biological Process	GO:0051402	neuron apoptotic process
Biological Process	GO:0030182	neuron differentiation
Biological Process	GO:0043065	positive regulation of apoptotic process
Biological Process	GO:0060545	positive regulation of necroptotic process
Biological Process	GO:0043525	positive regulation of neuron apoptotic process
Biological Process	GO:0045041	protein import into mitochondrial intermembrane space
Biological Process	GO:1902510	regulation of apoptotic DNA fragmentation
Biological Process	GO:0006979	response to oxidative stress

Reference

[1] Zhuo W, Zhang M, Tan J, Gao Y, Wang Y et al.. Lysine lactylation analysis of proteins in the heart of the Kawasaki disease mouse model.. Front Cell Dev Biol 13:1550220. 2025. PMID: 40114965.

[2] Wu D, Tang Y, Li X, Xiong S, Zhang Z et al.. Characterization of protein lactylation in healthy and ischemic mouse hearts.. Front Cardiovasc Med 12:1644886. 2025. PMID: 41089239.