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Overview

Uniprot IDQ9Z2Z6
Protein NameMitochondrial carnitine/acylcarnitine carrier protein
Gene NameSlc25a20
OrganismMus musculus

Kla Sites from experimental identification

Position Flanking peptide
157 SGTLDCAKKLYQEFG
158 GTLDCAKKLYQEFGI
244 FQTAPPGKYPNGFRD

Function

Mediates the electroneutral exchange of acylcarnitines (O-acyl-(R)-carnitine or L-acylcarnitine) of different acyl chain lengths (ranging from O-acetyl-(R)-carnitine to long-chain O-acyl-(R)-carnitines) with free carnitine ((R)-carnitine or L-carnitine) across the mitochondrial inner membrane, via a ping-pong mechanism. Key player in the mitochondrial oxidation pathway, it translocates the fatty acids in the form of acylcarnitines into the mitochondrial matrix, where the carnitine palmitoyltransferase 2 (CPT-2) activates them to undergo fatty acid beta-oxidation. Catalyzes the unidirectional transport (uniport) of carnitine at lower rates than the antiport (exchange)

Protein Sequence

10 MADEPKPISP 20 FKNLLAGGFG 30 GMCLVFVGHP 40 LDTVKVRLQT 50 QPPSLSGQPP 60 MYSGTLDCFR 70 KTLMREGITG 80 LYRGMAAPII 90 GVTPMFAVCF 100 FGFGLGKKLQ 110 QKSPEDELSY 120 PQLFTAGMLS 130 GVFTTGIMTP 140 GERIKCLLQI 150 QASSGENKYS 160 GTLDCAKKLY 170 QEFGIRGFYK 180 GTVLTLMRDV 190 PASGMYFMTY 200 EWLKNLFTPE 210 GKSVSDLSVP 220 RILVAGGFAG 230 IFNWAVAIPP 240 DVLKSRFQTA 250 PPGKYPNGFR 260 DVLRELIREE 270 GVTSLYKGFN 280 AVMIRAFPAN 290 AACFLGFEIA 300 MKFLNWIAPN L

Gene Ontology

Classification GO ID Description
Cellular Component GO:0005829 cytosol
Cellular Component GO:0005740 mitochondrial envelope
Cellular Component GO:0005743 mitochondrial inner membrane
Cellular Component GO:0005739 mitochondrion
Molecular Function GO:0005476 carnitine:O-acyl-L-carnitine antiporter activity
Molecular Function GO:0015227 O-acyl-L-carnitine transmembrane transporter activity
Biological Process GO:0006853 carnitine shuttle
Biological Process GO:1902603 carnitine transmembrane transport
Biological Process GO:0001701 in utero embryonic development
Biological Process GO:0006839 mitochondrial transport

Reference

[1] Zhuo W, Zhang M, Tan J, Gao Y, Wang Y et al.. Lysine lactylation analysis of proteins in the heart of the Kawasaki disease mouse model.. Front Cell Dev Biol 13:1550220. 2025. PMID: 40114965.

[2] Wu D, Tang Y, Li X, Xiong S, Zhang Z et al.. Characterization of protein lactylation in healthy and ischemic mouse hearts.. Front Cardiovasc Med 12:1644886. 2025. PMID: 41089239.