Subcellular Proteomic Mapping of Lysine Lactylation
PMID: 39569522
DOI: 10.1021/jasms.4c00366
Authors: Bao Q, Wan N, He Z, Cao J, Yuan W et al
Journal: Journal of the American Society for Mass Spectrometry (2024)
39569522_homo_sapiens — Homo sapiens
| Tissue or cell | HCT116 cells separated into cytoplasmic, nuclear, mitochondrial, and membrane fractions |
|---|---|
| Condition | Subcellular fractionation; DDA and DIA lactylome mapping |
| Enrichment method | Affinity enrichment after subcellular fractionation |
| MS instrument | Orbitrap Eclipse Tribrid |
| Search software | PEAKS Studio XPro; Spectronaut 19 |
| Localization threshold | DDA AScore ≥20; DIA PTM localization cutoff 0.75 |
| FDR | Target-decoy FDR 1% |
| Source-reported sites / proteins | 1217 / 553 |
| KlaDB-retained sites / proteins | 1091 / 495 |
| Evidence source | js4c00366_si_003.xlsx; Supplementary Table S3, Subcellular sheet |
| Curation notes | Source reports 1,217 Kla sites on 553 proteins. KlaDB retains only source-confirmed accession-position pairs present in the 64,072-site release; retained counts are shown separately.; Repository/accession: PXD053474; Additional criterion: Four subcellular fractions; 223 novel sites and 36 previously unreported proteins |